Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels

Yoichi Sasaki, Mio Oshikawa, Pankaj Bharmoria, Hironori Kouno, Akiko Hayashi-Takagi, Moritoshi Sato, Itsuki Ajioka, Nobuhiro Yanai, Nobuo Kimizuka

    研究成果: ジャーナルへの寄稿総説査読

    61 被引用数 (Scopus)

    抄録

    Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.

    本文言語英語
    ページ(範囲)17827-17833
    ページ数7
    ジャーナルAngewandte Chemie - International Edition
    58
    49
    DOI
    出版ステータス出版済み - 12月 2 2019

    All Science Journal Classification (ASJC) codes

    • 触媒
    • 化学 (全般)

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