Neofunctionalization of androgen receptor by Gain-of-Function mutations in teleost fish lineage

Yukiko Ogino, Shigehiro Kuraku, Hiroshi Ishibashi, Hitoshi Miyakawa, Eri Sumiya, Shinichi Miyagawa, Hajime Matsubara, Gen Yamada, Michael E. Baker, Taisen Iguchi

研究成果: Contribution to journalArticle査読

23 被引用数 (Scopus)

抄録

Steroid hormone receptor family provides an example of evolution of diverse transcription factors through wholegenome duplication (WGD). However, little is known about how their functions have been evolved after the duplication. Teleosts present a good model to investigate an accurate evolutionary history of protein function after WGD, because a teleost-specific WGD (TSGD) resulted in a variety of duplicated genes in modern fishes. This study focused on the evolution of androgen receptor (AR) gene, as two distinct paralogs, ARa and ARb, have evolved in teleost lineage after TSGD. ARa showed a unique intracellular localization with a higher transactivation response than that of ARb. Using site-directed mutagenesis and computational prediction of protein-ligand interactions, we identified two key substitutions generating a new functionality of euteleost ARa. The substitution in the hinge region contributes to the unique intracellular localization of ARa. The substitution on helices 10/11 in the ligand-binding domain possibly modulates hydrogen bonds that stabilize the receptor-ligand complex leading to the higher transactivation response of ARa. These substitutions were conserved in Acanthomorpha (spiny-rayed fish) ARas, but not in an earlier branching lineage among teleosts, Japanese eel. Insertion of these substitutions into ARs from Japanese eel recapitulates the evolutionary novelty of euteleost ARa. These findings together indicate that the substitutions generating a new functionality of teleost ARa were fixed in teleost genome after the divergence of the Elopomorpha lineage. Our findings provide a molecular explanation for an adaptation process leading to generation of the hyperactive AR subtype after TSGD.

本文言語英語
ページ(範囲)228-244
ページ数17
ジャーナルMolecular Biology and Evolution
33
1
DOI
出版ステータス出版済み - 1 1 2016
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生態、進化、行動および分類学
  • 分子生物学
  • 遺伝学

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