Nerve injury-activated microglia engulf myelinated axons in a P2Y12 signaling-dependent manner in the dorsal horn

Mitsuyo Maeda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue, Hiroshi Kiyama

研究成果: Contribution to journalArticle査読

56 被引用数 (Scopus)

抄録

The mechanisms underlying neuropathic pain are poorly understood. However, several studies have implied a role for reactive microglia located in the dorsal horn in neuropathic pain. To clarify the roles of activated microglia in neuropathic pain, we investigated the interactions among microglia and other neural components in the dorsal horn using electron microscopy. Microglia were more abundantly localized in layers II-III of the dorsal horn than in other areas, and some of them adhered to and engulfed both injured and uninjured myelinated axons. This microglial engulfment was rarely observed in the normal dorsal horn, and the number of microglia attached to myelinated axons was markedly increased on postoperative day 7 on the operated side. However, after blocking the P2Y12 ATP receptor in microglia by intrathecal administration of its antagonist, AR-C69931MX, the increase in the number of microglia attached to myelinated axons, as well as the development of tactile allodynia, were markedly suppressed, although the number of activated microglia did not change remarkably. These results indicate that engulfment of myelinated axons by activated microglia via P2Y12 signaling in the dorsal horn may be a critical event in the pathogenesis of neuropathic pain.

本文言語英語
ページ(範囲)1838-1846
ページ数9
ジャーナルGLIA
58
15
DOI
出版ステータス出版済み - 11 15 2010

All Science Journal Classification (ASJC) codes

  • 神経学
  • 細胞および分子神経科学

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