NEUROD1 is highly expressed in extensive-disease small cell lung cancer and promotes tumor cell migration

Yuki Ikematsu, Kentaro Tanaka, Gouji Toyokawa, Kayo Ijichi, Nobuhisa Ando, Yasuto Yoneshima, Eiji Iwama, Hiroyuki Inoue, Tetsuzo Tagawa, Yoichi Nakanishi, Isamu Okamoto

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Introduction: Small cell lung cancer (SCLC) manifests high-grade neuroendocrine features, and the transcription factors ASCL1 and NEUROD1 play an important role in the survival and growth as well as contribute to the heterogeneity of SCLC cells. The relative abundance of ASCL1 and NEUROD1 mRNAs differs among human SCLC cell lines, but the expression pattern of the encoded proteins in clinical SCLC specimens and its relation to clinicopathologic characteristics of patients have been unclear. Materials and Methods: We retrospectively analyzed tumor specimens collected from 95 previously untreated SCLC patients between June 1988 and December 2017 for ASCL1 and NEUROD1 expression by immunohistochemical staining. We also examined the effects of overexpression or depletion of NEUROD1 on cell migration in SCLC cell lines. Results: Overall survival did not differ significantly between SCLC patients with a high or low expression score for ASCL1 or NEUROD1 in their tumor samples. The staining score for NEUROD1 was significantly higher in extensive-disease (ED) samples than in limited-disease (LD) samples (median of 160 versus 80 out of a maximum of 300, P = 0.0389), and the proportion of tumors with an ASCL1highNEUROD1low phenotype was smaller for ED-SCLC. Overexpression or depletion of NEUROD1 in SCLC cell lines promoted or attenuated cell migratory activity, respectively. Conclusion: Our clinical and experimental data indicate that the expression of NEUROD1 is increased in ED-SCLC and promotes the migration of SCLC cells. NEUROD1 might thus contribute to metastasis in ED-SCLC.

本文言語英語
ページ(範囲)97-104
ページ数8
ジャーナルLung Cancer
146
DOI
出版ステータス出版済み - 8 2020

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 呼吸器内科
  • 癌研究

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