Neuron-specific expression of reporter gene in transgenic mice carrying the 5'-upstream region of mouse P/Q-type Ca2+ channel α(1A) subunit gene fused to E. coli lacZ reporter gene

Eiki Takahashi, Norimasa Miyamoto, Tohru Oki, Noriko Kajiwara, Keiko Furuya, Keiko Yanai-Taniguchi, Ken Ichi Yagami, Tetsuhiro Niidome, Isao Tanaka, Koichi Katayama

研究成果: ジャーナルへの寄稿学術誌査読

14 被引用数 (Scopus)

抄録

To dissect the molecular mechanisms underlying the neuron-specific expression of the P/Q type calcium channel α(1A) subunit gene, transgenic mice carrying a 0.5-kb, 1.5-kb, 3.0-kb or 6.3-kb 5'-upstream region of the gene fused to Escherichia coli lacZ reporter gene were produced. In transgenic mice carrying the 1.5-kb, 3.0-kb or 6.3-kb 5'-upstream region, the reporter gene was exclusively expressed in the nervous system, although those with the 0.5-kb 5'-upstream region failed to show reporter expression. Histological examinations showed that the three 5'-upstream regions induced distinct expression patterns of the reporter gene in the CNS and adrenal medulla. The 1.5-kb 5'-upstream region drove reporter gene expression in the olfactory bulb, dorsal cortex and hippocampus, while the regulatory element for the expression in the amygdaloid nucleus, septum, habenula medial nucleus, choroid plexus, substantia nigra, inferior colliculus, pontine nucleus and cerebellum was located in the 5'-upstream sequence between 1.5 kb and 6.3 kb. In the cerebellum, the expression of the reporter gene was induced by the 3.0-kb region in granule cells, whereas it was induced by the 6.3-kb region in Purkinje cells. The expression of the reporter gene in chromaffin cells in the adrenal medulla was induced only by the 6.3-kb 5'-upstream region. These results suggest that the expression of the mouse P/Q-type Ca2+ channel α(1A) subunit gene is regulated in a complex fashion by both positive and negative cis-regulatory elements. Copyright (C) 1999 Elsevier Science B.V.

本文言語英語
ページ(範囲)47-54
ページ数8
ジャーナルBrain Research
850
1-2
DOI
出版ステータス出版済み - 12月 11 1999
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

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