New archaeal viruses discovered by metagenomic analysis of viral communities in enrichment cultures

Ying Liu, David Brandt, Sonoko Ishino, Yoshizumi Ishino, Eugene V. Koonin, Jörn Kalinowski, Mart Krupovic, David Prangishvili

研究成果: ジャーナルへの寄稿記事

抄録

Viruses infecting hyperthermophilic archaea of the phylum Crenarchaeota display enormous morphological and genetic diversity, and are classified into 12 families. Eight of these families include only one or two species, indicating sparse sampling of the crenarchaeal virus diversity. In an attempt to expand the crenarchaeal virome, we explored virus diversity in the acidic, hot spring Umi Jigoku in Beppu, Japan. Environmental samples were used to establish enrichment cultures under conditions favouring virus replication. The host diversity in the enrichment cultures was restricted to members of the order Sulfolobales. Metagenomic sequencing of the viral communities yielded seven complete or near-complete double-stranded DNA virus genomes. Six of these genomes could be attributed to polyhedral and filamentous viruses that were observed by electron microscopy in the enrichment cultures. Two icosahedral viruses represented species in the family Portogloboviridae. Among the filamentous viruses, two were identified as new species in the families Rudiviridae and Lipothrixviridae, whereas two other formed a group seemingly distinct from the known virus genera. No particle morphotype could be unequivocally assigned to the seventh viral genome, which apparently represents a new virus type. Our results suggest that filamentous viruses are globally distributed and are prevalent virus types in extreme geothermal environments.

元の言語英語
ページ(範囲)2002-2014
ページ数13
ジャーナルEnvironmental Microbiology
21
発行部数6
DOI
出版物ステータス出版済み - 6 1 2019

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Archaeal Viruses
Metagenomics
enrichment culture
virus
Viruses
viruses
Lipothrixviridae
Rudiviridae
genome
Sulfolobales
Crenarchaeota
dsDNA viruses
analysis
Genome
Hot Springs
hot springs
DNA Viruses
Viral Genome
Archaea
Virus Replication

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

これを引用

New archaeal viruses discovered by metagenomic analysis of viral communities in enrichment cultures. / Liu, Ying; Brandt, David; Ishino, Sonoko; Ishino, Yoshizumi; Koonin, Eugene V.; Kalinowski, Jörn; Krupovic, Mart; Prangishvili, David.

:: Environmental Microbiology, 巻 21, 番号 6, 01.06.2019, p. 2002-2014.

研究成果: ジャーナルへの寄稿記事

Liu, Ying ; Brandt, David ; Ishino, Sonoko ; Ishino, Yoshizumi ; Koonin, Eugene V. ; Kalinowski, Jörn ; Krupovic, Mart ; Prangishvili, David. / New archaeal viruses discovered by metagenomic analysis of viral communities in enrichment cultures. :: Environmental Microbiology. 2019 ; 巻 21, 番号 6. pp. 2002-2014.
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