New detection method of biological targets based on HTS SQUID and magnetic marker

M. Ura, Y. Ueoka, K. Noguchi, K. Nakamura, Teruyoshi Sasayama, Takashi Yoshida, Keiji Enpuku

研究成果: 著書/レポートタイプへの貢献会議での発言

1 引用 (Scopus)

抄録

We developed improved methods of liquid-phase detection of biological targets using HTS SQUID and Brownian relaxation of magnetic markers. In a real sample, the aggregation and precipitation of free markers deteriorate their Brownian relaxation and cause so-called blank signals. In order to solve this problem, we developed two methods. First, we introduced a reaction field Bre during the binding reaction between the markers and targets. Using this method, we could obtain a strong signal from the bound markers without increasing the aggregation of the free markers. Next, we introduced a fieldreversal method in the measurement procedure in order to differentiate the signal from the markers in suspension from that from the precipitated markers. Using this procedure, we could eliminate the signal from the precipitated markers. Then, we detected biotin molecules by using these methods. The biotins were immobilized on the surfaces of large polymer beads with diameters of 3.3 μm and were detected with streptavidinconjugated magnetic markers. The minimum detectable molecular number concentration was 1.8 × 10-19mol/ml, indicating the high sensitivity of the present method.

元の言語英語
ホスト出版物のタイトル2015 15th International Superconductive Electronics Conference, ISEC 2015
出版者Institute of Electrical and Electronics Engineers Inc.
ISBN(電子版)9781467383486
DOI
出版物ステータス出版済み - 1 14 2016
イベント15th International Superconductive Electronics Conference, ISEC 2015 - Nagoya, 日本
継続期間: 7 6 20157 9 2015

その他

その他15th International Superconductive Electronics Conference, ISEC 2015
日本
Nagoya
期間7/6/157/9/15

Fingerprint

SQUIDs
Biotin
Agglomeration
Suspensions
Polymers
Molecules
Liquids

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

これを引用

Ura, M., Ueoka, Y., Noguchi, K., Nakamura, K., Sasayama, T., Yoshida, T., & Enpuku, K. (2016). New detection method of biological targets based on HTS SQUID and magnetic marker. : 2015 15th International Superconductive Electronics Conference, ISEC 2015 [7383474] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ISEC.2015.7383474

New detection method of biological targets based on HTS SQUID and magnetic marker. / Ura, M.; Ueoka, Y.; Noguchi, K.; Nakamura, K.; Sasayama, Teruyoshi; Yoshida, Takashi; Enpuku, Keiji.

2015 15th International Superconductive Electronics Conference, ISEC 2015. Institute of Electrical and Electronics Engineers Inc., 2016. 7383474.

研究成果: 著書/レポートタイプへの貢献会議での発言

Ura, M, Ueoka, Y, Noguchi, K, Nakamura, K, Sasayama, T, Yoshida, T & Enpuku, K 2016, New detection method of biological targets based on HTS SQUID and magnetic marker. : 2015 15th International Superconductive Electronics Conference, ISEC 2015., 7383474, Institute of Electrical and Electronics Engineers Inc., 15th International Superconductive Electronics Conference, ISEC 2015, Nagoya, 日本, 7/6/15. https://doi.org/10.1109/ISEC.2015.7383474
Ura M, Ueoka Y, Noguchi K, Nakamura K, Sasayama T, Yoshida T その他. New detection method of biological targets based on HTS SQUID and magnetic marker. : 2015 15th International Superconductive Electronics Conference, ISEC 2015. Institute of Electrical and Electronics Engineers Inc. 2016. 7383474 https://doi.org/10.1109/ISEC.2015.7383474
Ura, M. ; Ueoka, Y. ; Noguchi, K. ; Nakamura, K. ; Sasayama, Teruyoshi ; Yoshida, Takashi ; Enpuku, Keiji. / New detection method of biological targets based on HTS SQUID and magnetic marker. 2015 15th International Superconductive Electronics Conference, ISEC 2015. Institute of Electrical and Electronics Engineers Inc., 2016.
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AU - Ura, M.

AU - Ueoka, Y.

AU - Noguchi, K.

AU - Nakamura, K.

AU - Sasayama, Teruyoshi

AU - Yoshida, Takashi

AU - Enpuku, Keiji

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N2 - We developed improved methods of liquid-phase detection of biological targets using HTS SQUID and Brownian relaxation of magnetic markers. In a real sample, the aggregation and precipitation of free markers deteriorate their Brownian relaxation and cause so-called blank signals. In order to solve this problem, we developed two methods. First, we introduced a reaction field Bre during the binding reaction between the markers and targets. Using this method, we could obtain a strong signal from the bound markers without increasing the aggregation of the free markers. Next, we introduced a fieldreversal method in the measurement procedure in order to differentiate the signal from the markers in suspension from that from the precipitated markers. Using this procedure, we could eliminate the signal from the precipitated markers. Then, we detected biotin molecules by using these methods. The biotins were immobilized on the surfaces of large polymer beads with diameters of 3.3 μm and were detected with streptavidinconjugated magnetic markers. The minimum detectable molecular number concentration was 1.8 × 10-19mol/ml, indicating the high sensitivity of the present method.

AB - We developed improved methods of liquid-phase detection of biological targets using HTS SQUID and Brownian relaxation of magnetic markers. In a real sample, the aggregation and precipitation of free markers deteriorate their Brownian relaxation and cause so-called blank signals. In order to solve this problem, we developed two methods. First, we introduced a reaction field Bre during the binding reaction between the markers and targets. Using this method, we could obtain a strong signal from the bound markers without increasing the aggregation of the free markers. Next, we introduced a fieldreversal method in the measurement procedure in order to differentiate the signal from the markers in suspension from that from the precipitated markers. Using this procedure, we could eliminate the signal from the precipitated markers. Then, we detected biotin molecules by using these methods. The biotins were immobilized on the surfaces of large polymer beads with diameters of 3.3 μm and were detected with streptavidinconjugated magnetic markers. The minimum detectable molecular number concentration was 1.8 × 10-19mol/ml, indicating the high sensitivity of the present method.

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