New insights into the catalytic mechanism of Bombyx mori prostaglandin e synthase gained from structure-function analysis

Kohji Yamamoto, Mamoru Suzuki, Akifumi Higashiura, Kosuke Aritake, Yoshihiro Urade, Nobuko Uodome, Tofazzal Hossain, Atsushi Nakagawa

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Prostaglandin E synthase (PGES) catalyzes the isomerization of PGH 2 to PGE2. We previously reported the identification and structural characterization of Bombyx mori PGES (bmPGES), which belongs to Sigma-class glutathione transferase. Here, we extend these studies by determining the structure of bmPGES in complex with glutathione sulfonic acid (GTS) at a resolution of 1.37 Å using X-ray crystallography. GTS localized to the glutathione-binding site. We found that electron-sharing network of bmPGES includes Asn95, Asp96, and Arg98. Site-directed mutagenesis of these residues to create mutant forms of bmPGES mutants indicate that they contribute to catalytic activity. These results are, to our knowledge, the first to reveal the presence of an electron-sharing network in bmPGES.

本文言語英語
ページ(範囲)762-767
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
440
4
DOI
出版ステータス出版済み - 11 1 2013

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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