New mouse model of underactive bladder developed by placement of a metal ring around the bladder neck

Kareman Eljamal, Shunichi Kajioka, Tomoko Maki, Miho Ushijima, Kentaro Kawagoe, Ken Lee, Toshiyuki Sasaguri

研究成果: Contribution to journalArticle査読

抄録

Objective: To develop a new mouse model of underactive bladder (UAB) caused by chronic bladder outlet obstruction (BOO). Methods: BOO was created in 6-week-old male C57BL/6 mice using surgery to loosely place a silver jump ring around the bladder neck of each mouse. Micturition behavior (assessed with a metabolic cage) and cystometry were used to evaluate bladder function at 8 and 16 weeks after BOO. Following completion of the functional studies, the bladders of the mice were excised, weighed, and subjected to histological analysis. Results: Micturition behavior analysis showed that mice subjected to BOO for 16 weeks had a lower frequency of micturition (7.3 ± 1.1 vs 12.5 ± 3.0 times/d, P <.05) and volume per void (106.0 ± 0.1 vs 133.9 ± 3.2 μL, P <.05) than mice subjected to BOO for 8 weeks. Cystometry revealed that mice subjected to BOO for 16 weeks had lower baseline pressure (8.4 ± 0.6 vs 14.0 ± 0.7 cmH2O, P <.01) and micturition pressure (13.9 ± 1.1 vs 42.8 ± 1.7 cmH2O, P <.05) than mice subjected to BOO for 8 weeks. BOO caused progressive increases in bladder mass and collagen deposition over time. Conclusions: We successfully established a novel mouse model of UAB using surgery to place a silver jump ring loosely on the bladder neck. BOO initially induced bladder overactivity but subsequently resulted in UAB due to deterioration of detrusor smooth muscle contractility and progressive deposition of collagen in the bladder wall.

本文言語英語
ページ(範囲)299-307
ページ数9
ジャーナルLUTS: Lower Urinary Tract Symptoms
13
2
DOI
出版ステータス出版済み - 4 2021

All Science Journal Classification (ASJC) codes

  • 神経学
  • 泌尿器学

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