New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes

Kanji Okumoto, Tatsuaki Ono, Ryusuke Toyama, Ayako Shimomura, Aiko Nagata, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Microtubule-dependent long-distance movement of peroxisomes occurs in mammalian cells. However, its molecular mechanisms remain undefined. In this study, we identified three distinct splicing variants of human mitochondrial Rho GTPase-1 (Miro1), each containing amino acid sequence insertions 1 (named Miro1-var2), 2 (Miro1-var3), and both 1 and 2 (Miro1-var4), respectively, at upstream of the transmembrane domain. Miro1-var4 and Miro1-var2 are localized to peroxisomes in a manner dependent on the insertion 1 that is recognized by the cytosolic receptor Pex19p. Exogenous expression of Miro1-var4 induces accumulation of peroxisomes at the cell periphery and augments long-range movement of peroxisomes along microtubules. Depletion of all Miro1 variants by knocking down MIRO1 suppresses the long-distance movement of peroxisomes. Such abrogated movement is restored by reexpression of peroxisomal Miro1 variants. Collectively, our findings identify for the first time peroxisome-localized Miro1 variants as adapter proteins that link peroxisomes to the microtubule-dependent transport complexes including TRAK2 in the intracellular translocation of peroxisomes in mammalian cells.

元の言語英語
ページ(範囲)619-633
ページ数15
ジャーナルJournal of Cell Biology
217
発行部数2
DOI
出版物ステータス出版済み - 2 1 2018

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rho GTP-Binding Proteins
Peroxisomes
Microtubules
Insertional Mutagenesis
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes. / Okumoto, Kanji; Ono, Tatsuaki; Toyama, Ryusuke; Shimomura, Ayako; Nagata, Aiko; Fujiki, Yukio.

:: Journal of Cell Biology, 巻 217, 番号 2, 01.02.2018, p. 619-633.

研究成果: ジャーナルへの寄稿記事

Okumoto, K, Ono, T, Toyama, R, Shimomura, A, Nagata, A & Fujiki, Y 2018, 'New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes', Journal of Cell Biology, 巻. 217, 番号 2, pp. 619-633. https://doi.org/10.1083/jcb.201708122
Okumoto, Kanji ; Ono, Tatsuaki ; Toyama, Ryusuke ; Shimomura, Ayako ; Nagata, Aiko ; Fujiki, Yukio. / New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes. :: Journal of Cell Biology. 2018 ; 巻 217, 番号 2. pp. 619-633.
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