New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111

Hidemasa Izumiya, Yingxin Pei, Jun Terajima, Makoto Ohnishi, Tetsuya Hayashi, Sunao Iyoda, Haruo Watanabe

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

Enterohemorrhagic Escherichia coli (EHEC), a food- and waterborne pathogen, causes diarrhea, hemorrhagic colitis, and life-threatening HUS. MLVA is a newly developed and widely accepted genotyping tool. An MLVA system for EHEC O157 involving nine genomic loci has already been established. However, the present study revealed that the above-mentioned MLVA system cannot analyze EHEC O26 and O111 isolates-the second and third most dominant EHEC serogroups in Japan, respectively. Therefore, with several modifications to the O157 system and the use of nine additional loci, we developed an expanded MLVA system applicable to EHEC O26, O111, and O157. Our MLVA system had a relatively high resolution power for each of the three serogroups: Simpson's index of diversity was 0.991 (95% CI = 0.989-0.993), 0.988 (95% CI, 0.986-0.990), and 0.986 (95% CI, 0.979-0.993) for O26, O111, and O157, respectively. This systemalso detected outbreak-related isolates; the isolates collected during each of the 12 O26 and O111 outbreaks formed unique clusters, and most of the repeat copy numbers among the isolates collected during the sameoutbreak exhibited no or single-locus variations. These resultswere comparable to those of cluster analyses based on PFGE profiles. Therefore, our system can complement PFGE analysis-the current golden method. Because EHEC strains of three major serogroups can be rapidly analyzed on a single platformwith our expanded MLVA system, this systemcould be widely used in molecular epidemiological studies of EHEC infections.

本文言語英語
ページ(範囲)569-577
ページ数9
ジャーナルMICROBIOLOGY and IMMUNOLOGY
54
10
DOI
出版ステータス出版済み - 10 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • ウイルス学

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