Niche-specific adaptation of lactobacillus helveticus strains isolated from malt whisky and dairy fermentations

Yoshihiko Kido, Shintaro Maeno, Hiroki Tanno, Yuko Kichise, Yuh Shiwa, Akihito Endo

研究成果: Contribution to journalArticle査読

抄録

Lactobacillus helveticus is a well characterized lactobacillus for dairy fermentations that is also found in malt whisky fermenta-tions. The two environments contain considerable differences related to microbial growth, including the presence of different growth inhibitors and nutrients. The present study characterized L. helveticus strains originating from dairy fermentations (called milk strains hereafter) and malt whisky fermentations (called whisky strains hereafter) by in vitro phenotypic tests and comparative genomics. The whisky strains can tolerate ethanol more than the milk strains, whereas the milk strains can tolerate lysozyme and lactoferrin more than the whisky strains. Several plant-origin carbohydrates, including cellobiose, maltose, sucrose, fructooligosaccharide and salicin, were generally metabolized only by the whisky strains, whereas milk-derived carbo-hydrates, i.e. lactose and galactose, were metabolized only by the milk strains. Milk fermentation properties also distinguished the two groups. The general genomic characteristics, including genomic size, number of coding sequences and average nucleotide identity values, differentiated the two groups. The observed differences in carbohydrate metabolic properties between the two groups correlated with the presence of intact specific enzymes in glycoside hydrolase (GH) families GH1, GH4, GH13, GH32 and GH65. Several GHs in the milk strains were inactive due to the presence of stop codon(s) in genes encoding the GHs, and the inactivation patterns of the genes encoding specific enzymes assigned to GH1 in the milk strains suggested a possible diversification manner of L. helveticus strains. The present study has demonstrated how L. helveticus strains have adapted to their habitats.

本文言語英語
論文番号000560
ジャーナルMicrobial Genomics
7
4
DOI
出版ステータス出版済み - 2021
外部発表はい

All Science Journal Classification (ASJC) codes

  • 疫学
  • 微生物学
  • 分子生物学
  • 遺伝学

フィンガープリント

「Niche-specific adaptation of lactobacillus helveticus strains isolated from malt whisky and dairy fermentations」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル