Nitroxyl radicals-modified dendritic poly(l -lysine) as a contrast agent for Overhauser-enhanced MRI

Takuro Niidome, Risa Gokuden, Kazuto Watanabe, Takeshi Mori, Tatsuya Naganuma, Hideo Utsumi, Kazuhiro Ichikawa, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Overhauser-enhanced magnetic resonance imaging (OMRI), which is a double resonance technique, creates images of free radical distribution in animals by enhancing the water proton signal intensity by the overhauser effect. In this study, we constructed a contrast agent by combining PROXYL groups that have nitroxyl radicals with PEG-modified dendritic poly(l-lysine) that accumulates in the tumor by enhanced permeability and retention (EPR) effect. Addition of the PROXYL groups at the PEG chains termini on KG6 was advantageous in OMRI, because the ESR signal of the nitroxyl radical was maintained without decay caused by mobility restriction, even if the PROXYL groups were attached at 25 mol% on one molecule. After intramuscular injection of the molecule modified at 25 mol%, that is, PR25-PEG-KG6, a significant OMRI signal was observed at the injected site. However, no signal was detected in the tumor after intravenous injection of PR25-PEG-KG6 to a tumor-bearing mouse, although PR 25-PEG-KG6 itself accumulated in the tumor. The reason was that the nitroxyl radicals were immediately reduced in the blood after the injection, suggesting that use of stable nitroxyl radicals will enable detection of tumors by OMRI after intravenous injection.

元の言語英語
ページ(範囲)1425-1439
ページ数15
ジャーナルJournal of Biomaterials Science, Polymer Edition
25
発行部数13
DOI
出版物ステータス出版済み - 9 2 2014

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Magnetic resonance imaging
Contrast Media
Polyethylene glycols
Lysine
Tumors
Magnetic resonance
Magnetic Resonance Imaging
Imaging techniques
Neoplasms
Intravenous Injections
Bearings (structural)
Molecules
Intramuscular Injections
Free radicals
Free Radicals
Paramagnetic resonance
Protons
Permeability
Animals
Blood

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

これを引用

Nitroxyl radicals-modified dendritic poly(l -lysine) as a contrast agent for Overhauser-enhanced MRI. / Niidome, Takuro; Gokuden, Risa; Watanabe, Kazuto; Mori, Takeshi; Naganuma, Tatsuya; Utsumi, Hideo; Ichikawa, Kazuhiro; Katayama, Yoshiki.

:: Journal of Biomaterials Science, Polymer Edition, 巻 25, 番号 13, 02.09.2014, p. 1425-1439.

研究成果: ジャーナルへの寄稿記事

Niidome, Takuro ; Gokuden, Risa ; Watanabe, Kazuto ; Mori, Takeshi ; Naganuma, Tatsuya ; Utsumi, Hideo ; Ichikawa, Kazuhiro ; Katayama, Yoshiki. / Nitroxyl radicals-modified dendritic poly(l -lysine) as a contrast agent for Overhauser-enhanced MRI. :: Journal of Biomaterials Science, Polymer Edition. 2014 ; 巻 25, 番号 13. pp. 1425-1439.
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