Nlz1/Znf703 acts as a repressor of transcription

Mako Nakamura, Seong Kyu Choe, Alexander P. Runko, Paul D. Gardner, Charles G. Sagerström

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

Background. Members of the NET subfamily of zinc-finger proteins are related to the Sp-family of transcription factors and are required during embryogenesis. In particular, Nlz1/Znf703 and Nlz2/Znf503 are required for formation of rhombomere 4 of the vertebrate hindbrain. While NET family proteins have been hypothesized to regulate transcription, it remains unclear if they function as activators or repressors of transcription. Results. Here we demonstrate that Nlz proteins repress transcription both in cell lines and in developing zebrafish embryos. We first use standard cell culture-based reporter assays to demonstrate that Nlz1/Znf703 represses transcription of a luciferase reporter in four different cell lines. Structure-function analyses and pharmacological inhibition further reveal that Nlz1-mediated repression requires histone deacetylase activity. We next generate a stable transgenic zebrafish reporter line to demonstrate that Nlz1 promotes histone deacetylation at the transgenic promoter and repression of transgene expression during embryogenesis. Lastly, taking a genetic approach we find that endogenous Nlz proteins are required for formation of hindbrain rhombomere 4 during zebrafish embryogenesis by repressing expression of non-rhombomere 4 genes. Conclusion. We conclude that Nlz1/Znf703 acts as a repressor of transcription and hypothesize that other NET family members function in a similar manner.

元の言語英語
記事番号108
ジャーナルBMC Developmental Biology
8
DOI
出版物ステータス出版済み - 12 8 2008

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Zebrafish
Embryonic Development
Rhombencephalon
Sp Transcription Factors
Proteins
Activator Appliances
Cell Line
Histone Deacetylases
Zinc Fingers
Luciferases
Transgenes
Histones
Vertebrates
Embryonic Structures
Cell Culture Techniques
Pharmacology
Genes

All Science Journal Classification (ASJC) codes

  • Developmental Biology

これを引用

Nakamura, M., Choe, S. K., Runko, A. P., Gardner, P. D., & Sagerström, C. G. (2008). Nlz1/Znf703 acts as a repressor of transcription. BMC Developmental Biology, 8, [108]. https://doi.org/10.1186/1471-213X-8-108

Nlz1/Znf703 acts as a repressor of transcription. / Nakamura, Mako; Choe, Seong Kyu; Runko, Alexander P.; Gardner, Paul D.; Sagerström, Charles G.

:: BMC Developmental Biology, 巻 8, 108, 08.12.2008.

研究成果: ジャーナルへの寄稿記事

Nakamura, Mako ; Choe, Seong Kyu ; Runko, Alexander P. ; Gardner, Paul D. ; Sagerström, Charles G. / Nlz1/Znf703 acts as a repressor of transcription. :: BMC Developmental Biology. 2008 ; 巻 8.
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