NMR-based conformational analysis of sphingomyelin in bicelles

Toshiyuki Yamaguchi, Takashi Suzuki, Tomokazu Yasuda, Tohru Oishi, Nobuaki Matsumori, Michio Murata

研究成果: ジャーナルへの寄稿記事

29 引用 (Scopus)

抄録

Sphingomyelin (SM) is a common sphingolipid in mammalian membranes and is known to be substantially involved in cellular events such as the formation of lipid rafts. Despite its biological significance, conformation of SM in a membrane environment remains unclear because the noncrystalline property and anisotropic environment of lipid bilayers hampers the application of X-ray crystallography and NMR measurements. In this study, to elucidate the conformation of SM in membranes, we utilized bicelles as a substitute for a lipid bilayer membrane. First, we demonstrated through 31P NMR, 2H NMR, and dynamic light scattering experiments that SM forms both oriented and isotropic bicelles by changing the ratio of SM/dihexanoyl phosphatidylcholine. Then, we determined the conformation of SM in isotropic bicelles on the basis of coupling constants and NOE correlations in 1H NMR and found that the C2-C6 and amide groups of SM take a relatively rigid conformation in bicelles.

元の言語英語
ページ(範囲)270-278
ページ数9
ジャーナルBioorganic and Medicinal Chemistry
20
発行部数1
DOI
出版物ステータス出版済み - 1 1 2012

Fingerprint

Sphingomyelins
Nuclear magnetic resonance
Conformations
Lipid bilayers
Membranes
Lipid Bilayers
Sphingolipids
X ray crystallography
X Ray Crystallography
Dynamic light scattering
Membrane Lipids
Phosphatidylcholines
Amides
Lipids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

NMR-based conformational analysis of sphingomyelin in bicelles. / Yamaguchi, Toshiyuki; Suzuki, Takashi; Yasuda, Tomokazu; Oishi, Tohru; Matsumori, Nobuaki; Murata, Michio.

:: Bioorganic and Medicinal Chemistry, 巻 20, 番号 1, 01.01.2012, p. 270-278.

研究成果: ジャーナルへの寄稿記事

Yamaguchi, Toshiyuki ; Suzuki, Takashi ; Yasuda, Tomokazu ; Oishi, Tohru ; Matsumori, Nobuaki ; Murata, Michio. / NMR-based conformational analysis of sphingomyelin in bicelles. :: Bioorganic and Medicinal Chemistry. 2012 ; 巻 20, 番号 1. pp. 270-278.
@article{6c3f6fd77d214d8182764124334f39a7,
title = "NMR-based conformational analysis of sphingomyelin in bicelles",
abstract = "Sphingomyelin (SM) is a common sphingolipid in mammalian membranes and is known to be substantially involved in cellular events such as the formation of lipid rafts. Despite its biological significance, conformation of SM in a membrane environment remains unclear because the noncrystalline property and anisotropic environment of lipid bilayers hampers the application of X-ray crystallography and NMR measurements. In this study, to elucidate the conformation of SM in membranes, we utilized bicelles as a substitute for a lipid bilayer membrane. First, we demonstrated through 31P NMR, 2H NMR, and dynamic light scattering experiments that SM forms both oriented and isotropic bicelles by changing the ratio of SM/dihexanoyl phosphatidylcholine. Then, we determined the conformation of SM in isotropic bicelles on the basis of coupling constants and NOE correlations in 1H NMR and found that the C2-C6 and amide groups of SM take a relatively rigid conformation in bicelles.",
author = "Toshiyuki Yamaguchi and Takashi Suzuki and Tomokazu Yasuda and Tohru Oishi and Nobuaki Matsumori and Michio Murata",
year = "2012",
month = "1",
day = "1",
doi = "10.1016/j.bmc.2011.11.001",
language = "English",
volume = "20",
pages = "270--278",
journal = "Bioorganic and Medicinal Chemistry",
issn = "0968-0896",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - NMR-based conformational analysis of sphingomyelin in bicelles

AU - Yamaguchi, Toshiyuki

AU - Suzuki, Takashi

AU - Yasuda, Tomokazu

AU - Oishi, Tohru

AU - Matsumori, Nobuaki

AU - Murata, Michio

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Sphingomyelin (SM) is a common sphingolipid in mammalian membranes and is known to be substantially involved in cellular events such as the formation of lipid rafts. Despite its biological significance, conformation of SM in a membrane environment remains unclear because the noncrystalline property and anisotropic environment of lipid bilayers hampers the application of X-ray crystallography and NMR measurements. In this study, to elucidate the conformation of SM in membranes, we utilized bicelles as a substitute for a lipid bilayer membrane. First, we demonstrated through 31P NMR, 2H NMR, and dynamic light scattering experiments that SM forms both oriented and isotropic bicelles by changing the ratio of SM/dihexanoyl phosphatidylcholine. Then, we determined the conformation of SM in isotropic bicelles on the basis of coupling constants and NOE correlations in 1H NMR and found that the C2-C6 and amide groups of SM take a relatively rigid conformation in bicelles.

AB - Sphingomyelin (SM) is a common sphingolipid in mammalian membranes and is known to be substantially involved in cellular events such as the formation of lipid rafts. Despite its biological significance, conformation of SM in a membrane environment remains unclear because the noncrystalline property and anisotropic environment of lipid bilayers hampers the application of X-ray crystallography and NMR measurements. In this study, to elucidate the conformation of SM in membranes, we utilized bicelles as a substitute for a lipid bilayer membrane. First, we demonstrated through 31P NMR, 2H NMR, and dynamic light scattering experiments that SM forms both oriented and isotropic bicelles by changing the ratio of SM/dihexanoyl phosphatidylcholine. Then, we determined the conformation of SM in isotropic bicelles on the basis of coupling constants and NOE correlations in 1H NMR and found that the C2-C6 and amide groups of SM take a relatively rigid conformation in bicelles.

UR - http://www.scopus.com/inward/record.url?scp=84855204980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855204980&partnerID=8YFLogxK

U2 - 10.1016/j.bmc.2011.11.001

DO - 10.1016/j.bmc.2011.11.001

M3 - Article

C2 - 22133901

AN - SCOPUS:84855204980

VL - 20

SP - 270

EP - 278

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

SN - 0968-0896

IS - 1

ER -