NMR identification of the Tom20 binding segment in mitochondrial presequences

Takanori Muto, Takayuki Obita, Yoshito Abe, Toshihiro Shodai, Toshiya Endo, Daisuke Kohda

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Many mitochondrial proteins are synthesized in the cytosol as precursors with N-terminal presequences, and are imported into mitochondria with the aid of translocator protein complexes containing presequence-binding proteins. Tom20, a receptor protein which functions in an early step of the mitochondrial protein import, recognizes presequences with divergent amino acid sequences. Here, we report the identification of the segments involved in binding to Tom20 in mitochondrial presequences. We monitored the chemical shift perturbation of the NMR signals of five different 15N-labeled presequence peptides by the addition of the cytosolic receptor domain of rat or yeast Tom20. The perturbed segments occupy different positions, either near the N terminus or at the C terminus, in the presequences. Spin label experiments revealed that this is not due to different orientations of the presequence peptides bound to Tom20. The results presented here will offer a starting point to perform detailed analyses of Tom20-binding elements by systematic amino acid replacements.

元の言語英語
ページ(範囲)137-143
ページ数7
ジャーナルJournal of Molecular Biology
306
発行部数2
DOI
出版物ステータス出版済み - 2 16 2001
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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