NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae.

Kenji Ogura, Tsubasa Tandai, Sosuke Yoshinaga, Yoshihiro Kobashigawa, Hiroyuki Kumeta, Takashi Ito, Hideki Sumimoto, Fuyuhiko Inagaki

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Bem1 and Cdc24 of the budding yeast Saccharomyces cerevisiae interact with each other through PB1-PB1 heterodimer formation to regulate the establishment of cell polarity. Here we present the tertiary structure of the heterodimer of Bem1 and Cdc24 PB1 domains determined by NMR spectroscopy. To avoid ambiguity in the NMR spectral analysis, we first prepared a mutant of the Cdc24 PB1 domain that had truncated loops. The mutant provided well dispersed spectra without spectral overlapping, thus allowing unambiguous spectral assignments for structure determination. We confirmed that the loop deletion-mutant was quite similar to the wild-type in both 3D structure and binding affinity. The NMR structure of the heterodimer of the deletion-mutant of Cdc24 PB1 and Bem1 PB1 was determined using a variety of isotope labelled samples including perdeuteration. The interface between the Bem1/Cdc24 PB1 heterodimer was analysed at atomic resolution. Through a comparison with the tertiary structures of other PB1-PB1 heterodimers, we found that conserved electrostatic properties on the molecular surface were commonly used for PB1-PB1 interaction, but hydrophobic interactions were important for cognate interaction in Bem1/Cdc24 PB1 heterodimer formation.

元の言語英語
ページ(範囲)317-325
ページ数9
ジャーナルJournal of biochemistry
146
発行部数3
DOI
出版物ステータス出版済み - 1 1 2009

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Cell Polarity
Saccharomycetales
Static Electricity
Hydrophobic and Hydrophilic Interactions
Isotopes
Yeast
Saccharomyces cerevisiae
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Spectrum analysis
Nuclear magnetic resonance spectroscopy
Electrostatics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Ogura, K., Tandai, T., Yoshinaga, S., Kobashigawa, Y., Kumeta, H., Ito, T., ... Inagaki, F. (2009). NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae. Journal of biochemistry, 146(3), 317-325. https://doi.org/10.1093/jb/mvp075

NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae. / Ogura, Kenji; Tandai, Tsubasa; Yoshinaga, Sosuke; Kobashigawa, Yoshihiro; Kumeta, Hiroyuki; Ito, Takashi; Sumimoto, Hideki; Inagaki, Fuyuhiko.

:: Journal of biochemistry, 巻 146, 番号 3, 01.01.2009, p. 317-325.

研究成果: ジャーナルへの寄稿記事

Ogura, K, Tandai, T, Yoshinaga, S, Kobashigawa, Y, Kumeta, H, Ito, T, Sumimoto, H & Inagaki, F 2009, 'NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae.', Journal of biochemistry, 巻. 146, 番号 3, pp. 317-325. https://doi.org/10.1093/jb/mvp075
Ogura, Kenji ; Tandai, Tsubasa ; Yoshinaga, Sosuke ; Kobashigawa, Yoshihiro ; Kumeta, Hiroyuki ; Ito, Takashi ; Sumimoto, Hideki ; Inagaki, Fuyuhiko. / NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae. :: Journal of biochemistry. 2009 ; 巻 146, 番号 3. pp. 317-325.
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