Non-enzymatic covalent protein labeling using a reactive tag

Hiroshi Nonaka, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿記事

80 引用 (Scopus)

抄録

We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.

元の言語英語
ページ(範囲)15777-15779
ページ数3
ジャーナルJournal of the American Chemical Society
129
発行部数51
DOI
出版物ステータス出版済み - 12 26 2007

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Labeling
Proteins
Molecular Probes
Molecular recognition
Peptides
Experiments

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Non-enzymatic covalent protein labeling using a reactive tag. / Nonaka, Hiroshi; Tsukiji, Shinya; Ojida, Akio; Hamachi, Itaru.

:: Journal of the American Chemical Society, 巻 129, 番号 51, 26.12.2007, p. 15777-15779.

研究成果: ジャーナルへの寄稿記事

Nonaka, Hiroshi ; Tsukiji, Shinya ; Ojida, Akio ; Hamachi, Itaru. / Non-enzymatic covalent protein labeling using a reactive tag. :: Journal of the American Chemical Society. 2007 ; 巻 129, 番号 51. pp. 15777-15779.
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