Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

Shota Atsumi, Taizo Hanai, James C. Liao

研究成果: ジャーナルへの寄稿記事

1192 引用 (Scopus)

抄録

Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.

元の言語英語
ページ(範囲)86-89
ページ数4
ジャーナルNature
451
発行部数7174
DOI
出版物ステータス出版済み - 1 3 2008
外部発表Yes

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Biofuels
Alcohols
1-Butanol
Phenylethyl Alcohol
Wettability
Keto Acids
Metabolic Engineering
Glucose
Gasoline
Biosynthetic Pathways
Fermentation
Ethanol
Carbon
Escherichia coli
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

これを引用

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. / Atsumi, Shota; Hanai, Taizo; Liao, James C.

:: Nature, 巻 451, 番号 7174, 03.01.2008, p. 86-89.

研究成果: ジャーナルへの寄稿記事

Atsumi, Shota ; Hanai, Taizo ; Liao, James C. / Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. :: Nature. 2008 ; 巻 451, 番号 7174. pp. 86-89.
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