Non-invasive imaging of the levels and effects of glutathione on the redox status of mouse brain using electron paramagnetic resonance imaging

Miho C. Emoto, Yuta Matsuoka, Ken-Ichi Yamada, Hideo Sato-Akaba, Hirotada G. Fujii

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Glutathione (GSH) is the most abundant non-protein thiol that buffers reactive oxygen species in the brain. GSH does not reduce nitroxides directly, but in the presence of ascorbates, addition of GSH increases ascorbate-induced reduction of nitroxides. In this study, we used electron paramagnetic resonance (EPR) imaging and the nitroxide imaging probe, 3-methoxycarbonyl-2,2,5,5-tetramethyl-piperidine-1-oxyl (MCP), to non-invasively obtain spatially resolved redox data from mouse brains depleted of GSH with diethyl maleate compared to control. Based on the pharmacokinetics of the reduction reaction of MCP in the mouse heads, the pixel-based rate constant of its reduction reaction was calculated as an index of the redox status in vivo and mapped as a “redox map”. The obtained redox maps from control and GSH-depleted mouse brains showed a clear change in the brain redox status, which was due to the decreased levels of GSH in brains as measured by a biochemical assay. We observed a linear relationship between the reduction rate constant of MCP and the level of GSH for both control and GSH-depleted mouse brains. Using this relationship, the GSH level in the brain can be estimated from the redox map obtained with EPR imaging.

元の言語英語
ページ(範囲)802-806
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
485
発行部数4
DOI
出版物ステータス出版済み - 4 15 2017

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Electron Spin Resonance Spectroscopy
Oxidation-Reduction
Glutathione
Paramagnetic resonance
Brain
Imaging techniques
diethyl maleate
Rate constants
Pharmacokinetics
Sulfhydryl Compounds
Assays
Reactive Oxygen Species
Buffers
Pixels
Head

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Non-invasive imaging of the levels and effects of glutathione on the redox status of mouse brain using electron paramagnetic resonance imaging. / Emoto, Miho C.; Matsuoka, Yuta; Yamada, Ken-Ichi; Sato-Akaba, Hideo; Fujii, Hirotada G.

:: Biochemical and Biophysical Research Communications, 巻 485, 番号 4, 15.04.2017, p. 802-806.

研究成果: ジャーナルへの寄稿記事

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AU - Sato-Akaba, Hideo

AU - Fujii, Hirotada G.

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