Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions

Takahiro Matsui, Hiroki Mizuno, Takao Sudo, Junichi Kikuta, Naotsugu Haraguchi, Jun Ichiro Ikeda, Tsunekazu Mizushima, Hirofumi Yamamoto, Eiichi Morii, Masaki Mori, Masaru Ishii

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were 'conventional' images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images - the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) - rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness.

元の言語英語
記事番号6959
ジャーナルScientific reports
7
発行部数1
DOI
出版物ステータス出版済み - 12 1 2017

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Microscopy
Colorectal Neoplasms
Basement Membrane
Mucous Membrane
Fluorescence
Flavin-Adenine Dinucleotide
Hematoxylin
Eosine Yellowish-(YS)
NADP
NAD
Neoplasms
Epithelial Cells
Cell Membrane
Staining and Labeling
Pathologists

All Science Journal Classification (ASJC) codes

  • General

これを引用

Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions. / Matsui, Takahiro; Mizuno, Hiroki; Sudo, Takao; Kikuta, Junichi; Haraguchi, Naotsugu; Ikeda, Jun Ichiro; Mizushima, Tsunekazu; Yamamoto, Hirofumi; Morii, Eiichi; Mori, Masaki; Ishii, Masaru.

:: Scientific reports, 巻 7, 番号 1, 6959, 01.12.2017.

研究成果: ジャーナルへの寄稿記事

Matsui, T, Mizuno, H, Sudo, T, Kikuta, J, Haraguchi, N, Ikeda, JI, Mizushima, T, Yamamoto, H, Morii, E, Mori, M & Ishii, M 2017, 'Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions', Scientific reports, 巻. 7, 番号 1, 6959. https://doi.org/10.1038/s41598-017-07244-2
Matsui, Takahiro ; Mizuno, Hiroki ; Sudo, Takao ; Kikuta, Junichi ; Haraguchi, Naotsugu ; Ikeda, Jun Ichiro ; Mizushima, Tsunekazu ; Yamamoto, Hirofumi ; Morii, Eiichi ; Mori, Masaki ; Ishii, Masaru. / Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions. :: Scientific reports. 2017 ; 巻 7, 番号 1.
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