Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions

Takahiro Matsui, Hiroki Mizuno, Takao Sudo, Junichi Kikuta, Naotsugu Haraguchi, Jun Ichiro Ikeda, Tsunekazu Mizushima, Hirofumi Yamamoto, Eiichi Morii, Masaki Mori, Masaru Ishii

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were 'conventional' images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images - the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) - rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness.

本文言語英語
論文番号6959
ジャーナルScientific reports
7
1
DOI
出版ステータス出版済み - 12 1 2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • General

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