Non-specific esterase positive neutrophils in a case of refractory anemia with excess of blasts

Kazuhiko Nakamura, H. Ideguchi, T. Okajima, K. Kato

研究成果: ジャーナルへの寄稿記事

抄録

A 61-year-old woman was admitted with complaint of fever. The peripheral blood showed pancytopenia and bone marrow aspirate showed dysplasia in trilineage blood cells with increased blasts (18.2%). Bone marrow chromosome study revealed a karyotype of 46XX, -6, 3q-, +mar in 19 cells of 20 analyzed. She was diagnosed as refractory anemia with excess of blasts (RAEB). 95% of neutrophils in the bone marrow and 84% of that in the peripheral blood were stained with non-specific esterase using alpha-naphthyl acetate as substrate. On the other hand, the positivity of neutrophils for peroxidase. Sudan black B or chloroacetate esterase was markedly decreased. The phagocytotic activity of neutrophils was increased in comparison with normal control cells. Surface marker analysis of peripheral blood myeloid cells revealed increased expression of monocyte specific markers. These findings suggested that the patient's neutrophils, which were surely neutrophils in morphology, shared also monocyte-specific characters. After treatment with low dose Ara-C, pancytopenia was recovered and blasts in the bone marrow were reduced. Also was decreased non-specific esterase positive neutrophils, indicating that the neutrophils were derived from abnormal myeloid clone.

元の言語英語
ページ(範囲)386-390
ページ数5
ジャーナル[Rinsho ketsueki] The Japanese journal of clinical hematology
31
発行部数3
出版物ステータス出版済み - 3 1 1990

Fingerprint

Refractory Anemia with Excess of Blasts
Carboxylesterase
Neutrophils
Bone Marrow
Pancytopenia
Monocytes
Blood Cells
Mars
Cytarabine
Myeloid Cells
Karyotype
Peroxidase
Fever
Clone Cells
Chromosomes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

これを引用

Non-specific esterase positive neutrophils in a case of refractory anemia with excess of blasts. / Nakamura, Kazuhiko; Ideguchi, H.; Okajima, T.; Kato, K.

:: [Rinsho ketsueki] The Japanese journal of clinical hematology, 巻 31, 番号 3, 01.03.1990, p. 386-390.

研究成果: ジャーナルへの寄稿記事

Nakamura, Kazuhiko ; Ideguchi, H. ; Okajima, T. ; Kato, K. / Non-specific esterase positive neutrophils in a case of refractory anemia with excess of blasts. :: [Rinsho ketsueki] The Japanese journal of clinical hematology. 1990 ; 巻 31, 番号 3. pp. 386-390.
@article{cc9c8918a3d64211bb21ff3bd92088d5,
title = "Non-specific esterase positive neutrophils in a case of refractory anemia with excess of blasts",
abstract = "A 61-year-old woman was admitted with complaint of fever. The peripheral blood showed pancytopenia and bone marrow aspirate showed dysplasia in trilineage blood cells with increased blasts (18.2{\%}). Bone marrow chromosome study revealed a karyotype of 46XX, -6, 3q-, +mar in 19 cells of 20 analyzed. She was diagnosed as refractory anemia with excess of blasts (RAEB). 95{\%} of neutrophils in the bone marrow and 84{\%} of that in the peripheral blood were stained with non-specific esterase using alpha-naphthyl acetate as substrate. On the other hand, the positivity of neutrophils for peroxidase. Sudan black B or chloroacetate esterase was markedly decreased. The phagocytotic activity of neutrophils was increased in comparison with normal control cells. Surface marker analysis of peripheral blood myeloid cells revealed increased expression of monocyte specific markers. These findings suggested that the patient's neutrophils, which were surely neutrophils in morphology, shared also monocyte-specific characters. After treatment with low dose Ara-C, pancytopenia was recovered and blasts in the bone marrow were reduced. Also was decreased non-specific esterase positive neutrophils, indicating that the neutrophils were derived from abnormal myeloid clone.",
author = "Kazuhiko Nakamura and H. Ideguchi and T. Okajima and K. Kato",
year = "1990",
month = "3",
day = "1",
language = "English",
volume = "31",
pages = "386--390",
journal = "[Rinsho ketsueki] The Japanese journal of clinical hematology",
issn = "0485-1439",
publisher = "Nihon Rinsho Ketsueki Gakkai/Japan Society of Clinical Hematology",
number = "3",

}

TY - JOUR

T1 - Non-specific esterase positive neutrophils in a case of refractory anemia with excess of blasts

AU - Nakamura, Kazuhiko

AU - Ideguchi, H.

AU - Okajima, T.

AU - Kato, K.

PY - 1990/3/1

Y1 - 1990/3/1

N2 - A 61-year-old woman was admitted with complaint of fever. The peripheral blood showed pancytopenia and bone marrow aspirate showed dysplasia in trilineage blood cells with increased blasts (18.2%). Bone marrow chromosome study revealed a karyotype of 46XX, -6, 3q-, +mar in 19 cells of 20 analyzed. She was diagnosed as refractory anemia with excess of blasts (RAEB). 95% of neutrophils in the bone marrow and 84% of that in the peripheral blood were stained with non-specific esterase using alpha-naphthyl acetate as substrate. On the other hand, the positivity of neutrophils for peroxidase. Sudan black B or chloroacetate esterase was markedly decreased. The phagocytotic activity of neutrophils was increased in comparison with normal control cells. Surface marker analysis of peripheral blood myeloid cells revealed increased expression of monocyte specific markers. These findings suggested that the patient's neutrophils, which were surely neutrophils in morphology, shared also monocyte-specific characters. After treatment with low dose Ara-C, pancytopenia was recovered and blasts in the bone marrow were reduced. Also was decreased non-specific esterase positive neutrophils, indicating that the neutrophils were derived from abnormal myeloid clone.

AB - A 61-year-old woman was admitted with complaint of fever. The peripheral blood showed pancytopenia and bone marrow aspirate showed dysplasia in trilineage blood cells with increased blasts (18.2%). Bone marrow chromosome study revealed a karyotype of 46XX, -6, 3q-, +mar in 19 cells of 20 analyzed. She was diagnosed as refractory anemia with excess of blasts (RAEB). 95% of neutrophils in the bone marrow and 84% of that in the peripheral blood were stained with non-specific esterase using alpha-naphthyl acetate as substrate. On the other hand, the positivity of neutrophils for peroxidase. Sudan black B or chloroacetate esterase was markedly decreased. The phagocytotic activity of neutrophils was increased in comparison with normal control cells. Surface marker analysis of peripheral blood myeloid cells revealed increased expression of monocyte specific markers. These findings suggested that the patient's neutrophils, which were surely neutrophils in morphology, shared also monocyte-specific characters. After treatment with low dose Ara-C, pancytopenia was recovered and blasts in the bone marrow were reduced. Also was decreased non-specific esterase positive neutrophils, indicating that the neutrophils were derived from abnormal myeloid clone.

UR - http://www.scopus.com/inward/record.url?scp=0025392631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025392631&partnerID=8YFLogxK

M3 - Article

VL - 31

SP - 386

EP - 390

JO - [Rinsho ketsueki] The Japanese journal of clinical hematology

JF - [Rinsho ketsueki] The Japanese journal of clinical hematology

SN - 0485-1439

IS - 3

ER -