Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4.

Arisa Ohsaki, Kazuhiro Iiyama, Yoshitaka Miyagawa, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿学術誌査読

2 被引用数 (Scopus)

抄録

Nonhomologous end-joining (NHEJ) is one of the repair pathways for double-strand breaks (DSBs) in eukaryotic cells. By using linearized plasmid substrates, we have detected intramolecular NHEJ activity in a cell-free extract from the cultured silkworm cell line BmN4. The efficiency of NHEJ differed according to the structure of DNA ends; approximately 1% of input DNA was repaired when the substrate had cohesive ends. The reaction required the hydrolysis of nucleotide triphosphate; interestingly, all of four rNTPs or four dNTPs could support the reaction. A substrate with non-complementary DNA ends was mainly repaired by the DNA polymerase-mediated pathway. These results indicate that the present cell-free system will be useful to analyze the molecular mechanisms of DSB repair and NHEJ in insect cells.

本文言語英語
ページ(範囲)25-34
ページ数10
ジャーナルMolecular biology reports
32
1
DOI
出版ステータス出版済み - 3月 2005

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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