Noninvasive sensing of skin cancer for early diagnosis using cathespin L

Masaki Yamaguchi, Akira Date, Wataru Suzuki, Yusuke Tahara, Makoto Sasaki

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

It is reported that Cathepsin L (CatL) activity is correlated with actinic keratosis and squamous cell cancers. The purpose of this study is to propose an on-site testing of CatL activity in the skin, which can be used for the diagnosis of skin conditions noninvasively, easily, quickly and at any time. In this study, a novel immunochromatographic test-strip is proposed for CatL analysis in the skin. In order to realize the disposable test-strip, a colloidal gold technique was selected as the molecular recognition method for the CatL. A human skin sample was collected using tape stripping. The fabricated immuno-chromatographic test-strip (test-strip) consists of four pads, a sample pad, a conjugate release pad, a nitrocellulose membrane and an absorption pad. Based on optimal assay conditions, the sensitivity of the test-strip was measured. It required 90 s for a sample to reach the test-line on the test-strip. The optical density was proportion to the activity of CatL. In order to cancel out the influence of the collecting technique, the CatL/TP ratio was evaluated. It was suggested that the test-strip might be used for the qualitative analysis of CatL in the stratum corneum.

本文言語英語
ホスト出版物のタイトルProceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
ページ43-47
ページ数5
出版ステータス出版済み - 12 1 2010
外部発表はい
イベント7th IASTED International Conference on Biomedical Engineering, BioMED 2010 - Innsbruck, オーストリア
継続期間: 2 17 20102 19 2010

出版物シリーズ

名前Proceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
2

その他

その他7th IASTED International Conference on Biomedical Engineering, BioMED 2010
Countryオーストリア
CityInnsbruck
Period2/17/102/19/10

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine (miscellaneous)

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