Normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury.

Michael P. Hutchens, Richard J. Traystman, Tetsuhiro Fujiyoshi, Shin Nakayama, Paco S. Herson

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.

元の言語英語
ジャーナルJournal of visualized experiments : JoVE
発行部数54
出版物ステータス出版済み - 1 1 2011

Fingerprint

Resuscitation
Cardiopulmonary Resuscitation
Heart Arrest
Reperfusion Injury
Acute Kidney Injury
Anatomic Models
Genetic Techniques
Critical Illness
Reproduction
Rodentia
Animals
Ischemia
Animal Models
Costs and Cost Analysis
Mortality

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Normothermic cardiac arrest and cardiopulmonary resuscitation : a mouse model of ischemia-reperfusion injury. / Hutchens, Michael P.; Traystman, Richard J.; Fujiyoshi, Tetsuhiro; Nakayama, Shin; Herson, Paco S.

:: Journal of visualized experiments : JoVE, 番号 54, 01.01.2011.

研究成果: ジャーナルへの寄稿記事

@article{a09c5d85a86e4cb78c2b720d71853bab,
title = "Normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury.",
abstract = "Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.",
author = "Hutchens, {Michael P.} and Traystman, {Richard J.} and Tetsuhiro Fujiyoshi and Shin Nakayama and Herson, {Paco S.}",
year = "2011",
month = "1",
day = "1",
language = "English",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "54",

}

TY - JOUR

T1 - Normothermic cardiac arrest and cardiopulmonary resuscitation

T2 - a mouse model of ischemia-reperfusion injury.

AU - Hutchens, Michael P.

AU - Traystman, Richard J.

AU - Fujiyoshi, Tetsuhiro

AU - Nakayama, Shin

AU - Herson, Paco S.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.

AB - Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.

UR - http://www.scopus.com/inward/record.url?scp=84859926211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859926211&partnerID=8YFLogxK

M3 - Article

C2 - 21897359

AN - SCOPUS:84859926211

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 54

ER -