NOTCH4 is a potential therapeutic target for triple-negative breast cancer

Iori Nagamatsu, Hideya Ohnishi, Shojiro Matsushita, Makoto Kubo, Masaya Kai, Akira Imaizumi, kenji nakano, Masami Hattori, Yoshinao Oda, Masao Tanaka, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

25 引用 (Scopus)

抄録

Background/Aim: The prognosis for triple-negative breast cancer (TNBC) is poor. In the present study, we evaluated whether NOTCH4 receptor is a potential new therapeutic target for TNBC. Materials and Methods: In vitro proliferation and invasiveness were evaluated in TNBC cells with or without small-interfering RNA (siRNA) for NOTCH4, and with or without NOTCH4 plasmid transfection. In vivo, MDA-MB-231 cells with or without NOTCH4 siRNA were subcutaneously implanted into the flank regions of mice. The frequency of nuclear translocation of NOTCH4 was assessed by immunohistochemistry in 21 TNBC samples and 46 non-TNBC samples. Results: NOTCH4 inhibition in TNBC cells reduced proliferation and invasiveness, and NOTCH4 overexpression in TNBC cells increased proliferation and invasiveness. NOTCH4 inhibition reduced tumour volume and tumourigenicity of mouse xenografts. TNBC cells had a higher frequency of nuclear translocation of NOTCH4 than other cells. Conclusion: NOTCH4 is a new potential therapeutic target for triple-negative breast cancer.

元の言語英語
ページ(範囲)69-80
ページ数12
ジャーナルAnticancer research
34
発行部数1
出版物ステータス出版済み - 1 1 2014

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Triple Negative Breast Neoplasms
Therapeutics
Small Interfering RNA
Cell Proliferation
Tumor Burden
Heterografts
Transfection
Plasmids
Immunohistochemistry
Breast Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Nagamatsu, I., Ohnishi, H., Matsushita, S., Kubo, M., Kai, M., Imaizumi, A., ... Katano, M. (2014). NOTCH4 is a potential therapeutic target for triple-negative breast cancer. Anticancer research, 34(1), 69-80.

NOTCH4 is a potential therapeutic target for triple-negative breast cancer. / Nagamatsu, Iori; Ohnishi, Hideya; Matsushita, Shojiro; Kubo, Makoto; Kai, Masaya; Imaizumi, Akira; nakano, kenji; Hattori, Masami; Oda, Yoshinao; Tanaka, Masao; Katano, Mitsuo.

:: Anticancer research, 巻 34, 番号 1, 01.01.2014, p. 69-80.

研究成果: ジャーナルへの寄稿記事

Nagamatsu, I, Ohnishi, H, Matsushita, S, Kubo, M, Kai, M, Imaizumi, A, nakano, K, Hattori, M, Oda, Y, Tanaka, M & Katano, M 2014, 'NOTCH4 is a potential therapeutic target for triple-negative breast cancer', Anticancer research, 巻. 34, 番号 1, pp. 69-80.
Nagamatsu, Iori ; Ohnishi, Hideya ; Matsushita, Shojiro ; Kubo, Makoto ; Kai, Masaya ; Imaizumi, Akira ; nakano, kenji ; Hattori, Masami ; Oda, Yoshinao ; Tanaka, Masao ; Katano, Mitsuo. / NOTCH4 is a potential therapeutic target for triple-negative breast cancer. :: Anticancer research. 2014 ; 巻 34, 番号 1. pp. 69-80.
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abstract = "Background/Aim: The prognosis for triple-negative breast cancer (TNBC) is poor. In the present study, we evaluated whether NOTCH4 receptor is a potential new therapeutic target for TNBC. Materials and Methods: In vitro proliferation and invasiveness were evaluated in TNBC cells with or without small-interfering RNA (siRNA) for NOTCH4, and with or without NOTCH4 plasmid transfection. In vivo, MDA-MB-231 cells with or without NOTCH4 siRNA were subcutaneously implanted into the flank regions of mice. The frequency of nuclear translocation of NOTCH4 was assessed by immunohistochemistry in 21 TNBC samples and 46 non-TNBC samples. Results: NOTCH4 inhibition in TNBC cells reduced proliferation and invasiveness, and NOTCH4 overexpression in TNBC cells increased proliferation and invasiveness. NOTCH4 inhibition reduced tumour volume and tumourigenicity of mouse xenografts. TNBC cells had a higher frequency of nuclear translocation of NOTCH4 than other cells. Conclusion: NOTCH4 is a new potential therapeutic target for triple-negative breast cancer.",
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AU - Ohnishi, Hideya

AU - Matsushita, Shojiro

AU - Kubo, Makoto

AU - Kai, Masaya

AU - Imaizumi, Akira

AU - nakano, kenji

AU - Hattori, Masami

AU - Oda, Yoshinao

AU - Tanaka, Masao

AU - Katano, Mitsuo

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N2 - Background/Aim: The prognosis for triple-negative breast cancer (TNBC) is poor. In the present study, we evaluated whether NOTCH4 receptor is a potential new therapeutic target for TNBC. Materials and Methods: In vitro proliferation and invasiveness were evaluated in TNBC cells with or without small-interfering RNA (siRNA) for NOTCH4, and with or without NOTCH4 plasmid transfection. In vivo, MDA-MB-231 cells with or without NOTCH4 siRNA were subcutaneously implanted into the flank regions of mice. The frequency of nuclear translocation of NOTCH4 was assessed by immunohistochemistry in 21 TNBC samples and 46 non-TNBC samples. Results: NOTCH4 inhibition in TNBC cells reduced proliferation and invasiveness, and NOTCH4 overexpression in TNBC cells increased proliferation and invasiveness. NOTCH4 inhibition reduced tumour volume and tumourigenicity of mouse xenografts. TNBC cells had a higher frequency of nuclear translocation of NOTCH4 than other cells. Conclusion: NOTCH4 is a new potential therapeutic target for triple-negative breast cancer.

AB - Background/Aim: The prognosis for triple-negative breast cancer (TNBC) is poor. In the present study, we evaluated whether NOTCH4 receptor is a potential new therapeutic target for TNBC. Materials and Methods: In vitro proliferation and invasiveness were evaluated in TNBC cells with or without small-interfering RNA (siRNA) for NOTCH4, and with or without NOTCH4 plasmid transfection. In vivo, MDA-MB-231 cells with or without NOTCH4 siRNA were subcutaneously implanted into the flank regions of mice. The frequency of nuclear translocation of NOTCH4 was assessed by immunohistochemistry in 21 TNBC samples and 46 non-TNBC samples. Results: NOTCH4 inhibition in TNBC cells reduced proliferation and invasiveness, and NOTCH4 overexpression in TNBC cells increased proliferation and invasiveness. NOTCH4 inhibition reduced tumour volume and tumourigenicity of mouse xenografts. TNBC cells had a higher frequency of nuclear translocation of NOTCH4 than other cells. Conclusion: NOTCH4 is a new potential therapeutic target for triple-negative breast cancer.

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