Novel antibacterial compounds specifically targeting the essential WalR response regulator

Yasuhiro Gotoh, Akihiro Doi, Eiji Furuta, Sarah Dubrac, Yoshimasa Ishizaki, Masato Okada, Masayuki Igarashi, Norihiko Misawa, Hirofumi Yoshikawa, Toshihide Okajima, Tarek Msadek, Ryutaro Utsumi

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抜粋

The WalK/WalR (YycG/YycF) two-component system, which is essential for cell viability, is highly conserved and specific to low-GC percentage of Gram-positive bacteria, making it an attractive target for novel antimicrobial compounds. Recent work has shown that WalK/WalR exerts an effect as a master regulatory system in controlling and coordinating cell wall metabolism with cell division in Bacillus subtilis and Staphylococcus aureus. In this paper, we develop a high-throughput screening system for WalR inhibitors and identify two novel inhibitors targeting the WalR response regulator (RR): walrycin A (4-methoxy-1-naphthol) and walrycin B (1,6-dimethyl-3-4-(trifluoromethyl) phenylpyrimido5,4-e1,2,4triazine-5,7-dione). Addition of these compounds simultaneously affects the expression of WalR regulon genes, leading to phenotypes consistent with those of cells starved for the WalK/WalR system and having a bactericidal effect. B. subtilis cells form extremely long aseptate filaments and S. aureus cells form large aggregates under these conditions. These results show that walrycins A and B are the first antibacterial agents targeting WalR in B. subtilis and S. aureus.

元の言語英語
ページ(範囲)127-134
ページ数8
ジャーナルJournal of Antibiotics
63
発行部数3
DOI
出版物ステータス出版済み - 3 1 2010
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

これを引用

Gotoh, Y., Doi, A., Furuta, E., Dubrac, S., Ishizaki, Y., Okada, M., ... Utsumi, R. (2010). Novel antibacterial compounds specifically targeting the essential WalR response regulator. Journal of Antibiotics, 63(3), 127-134. https://doi.org/10.1038/ja.2010.4