Novel biosensor for the rapid measurement of estrogen based on a ligand-receptor interaction

M. Murata, M. Nakayama, H. Irie, K. Yakabe, K. Fukuma, Y. Katayama, M. Maeda

研究成果: ジャーナルへの寄稿記事

40 引用 (Scopus)

抄録

A bioaffinity sensor was developed aiming at the detection of estrogen. This biosensor system is based on the specific binding of estrogen to its receptor immobilized on a gold disk electrode. The recombinant DNA encoding human estrogen receptor ligand-binding domain was expressed in bacteria using the histidine-tag fusion system. The expression of the fusion protein was under control of a bacteriophage T7 promoter, and the protein was purified under native conditions by affinity chromatography, which is based on a specific interaction between a histidine-tag, located in the N-terminus of the protein, and the Ni(II) chelate adsorbent. The protein was immobilized on an Au-electrode with Ni(II)-mediated chemisorption using a histidine tag and thiol-modified iminodiacetic acid. Cyclic voltammetric measurements showed that the reversible electrochemical reaction of a ferrocyanide/ferricyanide redox couple was suppressed by the presence of estrogen in a concentration-dependent manner. It seems reasonable to suppose that the electrostatic property of the protein layer on the electrode surface was altered by complexation with estrogen. These data suggest that this biosensor is applicable to the evaluation binding activities of the chemicals toward the human estrogen receptor.

元の言語英語
ページ(範囲)387-390
ページ数4
ジャーナルanalytical sciences
17
発行部数3
DOI
出版物ステータス出版済み - 1 1 2001

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Biosensors
Estrogens
Histidine
Ligands
Estrogen Receptors
Electrodes
Proteins
Fusion reactions
Gi-Go GTP-Binding Protein alpha Subunits
Affinity chromatography
Bacteriophages
Recombinant DNA
Chemisorption
Complexation
Sulfhydryl Compounds
Gold
Adsorbents
Electrostatics
Bacteria
Sensors

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Novel biosensor for the rapid measurement of estrogen based on a ligand-receptor interaction. / Murata, M.; Nakayama, M.; Irie, H.; Yakabe, K.; Fukuma, K.; Katayama, Y.; Maeda, M.

:: analytical sciences, 巻 17, 番号 3, 01.01.2001, p. 387-390.

研究成果: ジャーナルへの寄稿記事

Murata, M, Nakayama, M, Irie, H, Yakabe, K, Fukuma, K, Katayama, Y & Maeda, M 2001, 'Novel biosensor for the rapid measurement of estrogen based on a ligand-receptor interaction', analytical sciences, 巻. 17, 番号 3, pp. 387-390. https://doi.org/10.2116/analsci.17.387
Murata, M. ; Nakayama, M. ; Irie, H. ; Yakabe, K. ; Fukuma, K. ; Katayama, Y. ; Maeda, M. / Novel biosensor for the rapid measurement of estrogen based on a ligand-receptor interaction. :: analytical sciences. 2001 ; 巻 17, 番号 3. pp. 387-390.
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