Novel Distinctive Roles of Docking Proteins in Short-term Synaptic Plasticity of Frog Neuromuscular Transmission Revealed by Botulinum Neurotoxins

Yasuhiro Imafuku, Koh ichi Enomoto, Hiroko Kataoka, Isao Ito, Takashi Maeno

研究成果: ジャーナルへの寄稿記事

抄録

Short-term synaptic plasticity (SSP) is a basic mechanism for temporal processing of neural information in synaptic transmission. Facilitation, the fastest component of SSP, has been extensively investigated with regard to Ca 2+ signaling and other relevant substances. However, systematic analyses on the slower components of SSP, originated by Magleby and Zengel, have remained stagnant for decades, as few chemicals directly modifying these slower components have been identified. In combination with refined experimental protocols designed to study the stimulation frequency-dependence of SSP and botulinum neurotoxins A and C (BoNT-A and BoNT-C), we investigated SSP of frog neuromuscular transmission to clarify the roles of synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin, SNARE proteins exclusively participating in vesicular events including docking, priming and exocytosis. We found that BoNT-A treatment eliminated slow potentiation, and BoNT-C poisoning abolished intermediate augmentation, two components of SSP. Fast facilitation was maintained after double poisoning with BoNT-A and -C, but the postsynaptic response became biphasic. A novel depression, termed repression, emerged by double poisoning. Repression was different from depletion because it developed even at a low-frequency stimulation of 1 Hz. We conclude that SNAP-25 and syntaxin not only play roles as cooperative exocytotic machinery, but also have roles in SSP.

元の言語英語
ページ(範囲)374-385
ページ数12
ジャーナルNeuroscience
369
DOI
出版物ステータス出版済み - 1 15 2018

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Neuronal Plasticity
Neurotoxins
Anura
Synaptosomal-Associated Protein 25
Proteins
Poisoning
Qa-SNARE Proteins
SNARE Proteins
Type A Botulinum Toxins
Exocytosis
Automatic Data Processing
Synaptic Transmission
incobotulinumtoxinA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Novel Distinctive Roles of Docking Proteins in Short-term Synaptic Plasticity of Frog Neuromuscular Transmission Revealed by Botulinum Neurotoxins. / Imafuku, Yasuhiro; Enomoto, Koh ichi; Kataoka, Hiroko; Ito, Isao; Maeno, Takashi.

:: Neuroscience, 巻 369, 15.01.2018, p. 374-385.

研究成果: ジャーナルへの寄稿記事

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AU - Ito, Isao

AU - Maeno, Takashi

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