Novel DNA/polymer conjugate for intelligent antisense reagent with improved nuclease resistance

Masaharu Murata, Wataru Kaku, Takahisa Anada, Yoshikuni Sato, Takeshi Kano, Mizuo Maeda, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

Antisense technology provides an effective strategy to inhibit synthesis of the gene product. We prepared a novel antisense reagent comprised of oligodeoxynucleotides (ODN) and a thermo responsive polymer, poly(N-isopropylacrylamide) (PNIPAAm). The conjugate inhibited gene expression in a dose-dependent manner. The ODN-PNIPAAm conjugate demonstrated excellent resistance to S1 nuclease. In particular, PNIPAAm-modified antisense ODN at the 3′,5′-ends of the ODN provided complete resistance against nuclease at 37°C, which is above the phase transition temperature of the PNIPAAm side chain. These characteristics of the conjugate suggest it may have potential for use in a new gene delivery system as part of an antisense strategy.

元の言語英語
ページ(範囲)3967-3970
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
13
発行部数22
DOI
出版物ステータス出版済み - 11 17 2003

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Oligodeoxyribonucleotides
Polymers
DNA
Genes
Gene Transfer Techniques
Transition Temperature
Phase Transition
Gene expression
Phase transitions
Technology
Gene Expression
poly-N-isopropylacrylamide

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Novel DNA/polymer conjugate for intelligent antisense reagent with improved nuclease resistance. / Murata, Masaharu; Kaku, Wataru; Anada, Takahisa; Sato, Yoshikuni; Kano, Takeshi; Maeda, Mizuo; Katayama, Yoshiki.

:: Bioorganic and Medicinal Chemistry Letters, 巻 13, 番号 22, 17.11.2003, p. 3967-3970.

研究成果: ジャーナルへの寄稿記事

Murata, Masaharu ; Kaku, Wataru ; Anada, Takahisa ; Sato, Yoshikuni ; Kano, Takeshi ; Maeda, Mizuo ; Katayama, Yoshiki. / Novel DNA/polymer conjugate for intelligent antisense reagent with improved nuclease resistance. :: Bioorganic and Medicinal Chemistry Letters. 2003 ; 巻 13, 番号 22. pp. 3967-3970.
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AU - Maeda, Mizuo

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