Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I

N. Yamazaki, Y. Shinohara, K. Kajimoto, M. Shindo, H. Terada

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

For characterization of the detailed gene structure of human muscle type carnitine palmitoyltransferase I (M-CPTI), we analyzed the 5'-upstream region of the M-CPTI transcripts. As a result, we found a cDNA clone containing a nucleotide sequence unexpected from the reported M-CPTI gene structure in the upstream region of its 5' end. Comparison of this nucleotide sequence with that of genomic DNA showed that this sequence was derived from the 3'-untranslated region of the gene encoding choline/ethanolamine kinase-β (CK/EK-β) located upstream of the M-CPTI gene. Southern blot analysis showed that there was no other region homologous to the CK/EK-β gene in the whole human genome. Thus, the overlapping transcript was concluded to be produced from the functional genes of CK/EK-β and M-CPTI. Furthermore, cDNAs containing both exons of these genes were detected by the polymerase chain reaction using the cDNA of human heart M-CPTI obtained by specific reverse transcription from its 3'-untranslated region as a template. From these results, the production and organization of these overlapping transcripts are discussed.

本文言語英語
ページ(範囲)31739-31746
ページ数8
ジャーナルJournal of Biological Chemistry
275
41
DOI
出版ステータス出版済み - 10月 13 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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