Novel G Proteins, Rag C and Rag D, Interact with GTP-binding Proteins, Rag A and Rag B

Takeshi Sekiguchi, Eiji Hirose, Nobutaka Nakashima, Miki Ii, Takeharu Nishimoto

研究成果: Contribution to journalArticle査読

159 被引用数 (Scopus)

抄録

Rag A/Gtr1p are G proteins and are known to be involved in the RCC1-Ran pathway. We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. Rag C demonstrates homology with Rag D (81.1% identity) and with Gtr2p of Saccharomyces cerevisiae (46.1% identity), and it belongs to the Rag A subfamily of the Ras family. Rag C and Rag D contain conserved GTP-binding motifs (PM-1, -2, and -3) in their N-terminal regions. Recombinant glutathione S-transferase fusion protein of Rag C efficiently bound to both [ 3H]GTP and [3H]GDP. Rag A was associated with both Rag C and Rag D in their C-terminal regions where a potential leucine zipper motif and a coiled-coil structure were found. Rag C and D were associated with both the GDP and GTP forms of Rag A. Both Rag C and Rag D changed their subcellular localization, depending on the nucleotide-bound state of Rag A. In a similar way, the disruption of S. cerevisiae GTR1 resulted in a change in the localization of Gtr2p.

本文言語英語
ページ(範囲)7246-7257
ページ数12
ジャーナルJournal of Biological Chemistry
276
10
DOI
出版ステータス出版済み - 3 9 2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Novel G Proteins, Rag C and Rag D, Interact with GTP-binding Proteins, Rag A and Rag B」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル