Novel Leu-Lys-specific peptidase (leulysin) produced by gel-entrapped yeast cells

Tsutomu Okada, Kenji Sonomoto, Atsuo Tanaka

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

Saccharomyces cerevisiae cells entrapped with a neutral hydrophilic photo-crosslinkable resin prepolymers specifically excreted into a cultured medium a new type of a peptidase, which cleaved Leu-Lys bond of α-mating factor. The enzyme was purified by membrane filtration followed by ion exchange chromatography and gel filtration chromatography. The purified enzyme showed a strict substrate specificity on internal Leu-Lys bond. Leu-Lys bond near terminus of a molecule and Leu-X and X-Lys bonds examined so far were not hydrolyzed by the enzyme.

本文言語英語
ページ(範囲)316-322
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
145
1
DOI
出版ステータス出版済み - 5 29 1987

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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