Novel modular domain PB1 recognizes pc motif to mediate functional protein-protein interactions

Takashi Ito, Yasushi Matsui, Tetsuro Ago, Kazuhisa Ota, Hideki Sumimoto

研究成果: ジャーナルへの寄稿記事

124 引用 (Scopus)

抄録

Modular domains mediating specific protein-protein interactions play central roles in the formation of complex regulatory networks to execute various cellular activities. Here we identify a novel domain PB1 in the budding yeast protein Bem1p, which functions in polarity establishment, and mammalian p67phox, which activates the microbicidal phagocyte NADPH oxidase. Each of these specifically recognizes an evolutionarily conserved PC motif to interact directly with Cdc24p (an essential protein for cell polarization) and p40phox (a component of the signaling complex for the oxidase), respectively. Swapping the PB1 domain of Bem1p with that of p67phox, which abolishes its interaction with Cdc24p, confers on cells temperature-sensitive growth and a bilateral mating defect. These phenotypes are suppressed by a mutant Cdc24p harboring the PC motif-containing region of p40phox, which restores the interaction with the altered Bem1p. This domain-swapping experiment demonstrates that Bem1p function requires interaction with Cdc24p, in which the PB1 domain and the PC motif participate as responsible modules.

元の言語英語
ページ(範囲)3938-3946
ページ数9
ジャーナルEMBO Journal
20
発行部数15
DOI
出版物ステータス出版済み - 8 1 2001

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Proteins
Saccharomycetales
Fungal Proteins
NADPH Oxidase
Phagocytes
Oxidoreductases
Polarization
Phenotype
Defects
Temperature
Growth
Experiments
neutrophil cytosol factor 40K
neutrophil cytosol factor 67K

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Novel modular domain PB1 recognizes pc motif to mediate functional protein-protein interactions. / Ito, Takashi; Matsui, Yasushi; Ago, Tetsuro; Ota, Kazuhisa; Sumimoto, Hideki.

:: EMBO Journal, 巻 20, 番号 15, 01.08.2001, p. 3938-3946.

研究成果: ジャーナルへの寄稿記事

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