Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger

Kaoru Takegawa, A. Konjo, H. Iwamoto, K. Fujiwara, Y. Hosokawa, I. Kato, K. Hiromi, S. Iwahara

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

The primary structure of the N-linked sugar chains of glucose oxidase from Aspergillus niger was investigated. These sugar chains were released from the polypeptide backbone by hydrazinolysis, and the reducing ends of the sugar chains were pyridylaminated. HPLC of the pyridylamino sugar chains with an amide-silica column showed at least seven sugar chain peaks. Chemical and exoglycosidase, digestion and 400MHz 1H-NMR studies of the sugar chains of lower molecular weight showed that these were novel oligomannose-type sugar chains, (Man)5-7(GlcNAc)2, with the structure: ±Manα1→3Manα1→3(Manα1→6)Manα1→6 (±Manα1→3Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc.

元の言語英語
ページ(範囲)181-190
ページ数10
ジャーナルBiochemistry international
25
発行部数1
出版物ステータス出版済み - 12 1 1991

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Glucose Oxidase
Aspergillus niger
Glycoside Hydrolases
Aspergillus
Amides
Sugars
Silicon Dioxide
Digestion
Molecular Weight
High Pressure Liquid Chromatography
Peptides
N,N-diacetylchitobiose
Proton Magnetic Resonance Spectroscopy
Molecular weight
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

Takegawa, K., Konjo, A., Iwamoto, H., Fujiwara, K., Hosokawa, Y., Kato, I., ... Iwahara, S. (1991). Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger. Biochemistry international, 25(1), 181-190.

Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger. / Takegawa, Kaoru; Konjo, A.; Iwamoto, H.; Fujiwara, K.; Hosokawa, Y.; Kato, I.; Hiromi, K.; Iwahara, S.

:: Biochemistry international, 巻 25, 番号 1, 01.12.1991, p. 181-190.

研究成果: ジャーナルへの寄稿記事

Takegawa, K, Konjo, A, Iwamoto, H, Fujiwara, K, Hosokawa, Y, Kato, I, Hiromi, K & Iwahara, S 1991, 'Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger', Biochemistry international, 巻. 25, 番号 1, pp. 181-190.
Takegawa K, Konjo A, Iwamoto H, Fujiwara K, Hosokawa Y, Kato I その他. Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger. Biochemistry international. 1991 12 1;25(1):181-190.
Takegawa, Kaoru ; Konjo, A. ; Iwamoto, H. ; Fujiwara, K. ; Hosokawa, Y. ; Kato, I. ; Hiromi, K. ; Iwahara, S. / Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger. :: Biochemistry international. 1991 ; 巻 25, 番号 1. pp. 181-190.
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