Novel styrylbenzene derivatives for detecting amyloid deposits

Manabu Nakazono, Konen Obayashi, Kazumi Sasamoto, Katsumi Tomiyoshi, Genki Suenaga, Yukio Ando

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Background: Various styrylbenzene compounds were synthesized and evaluated as mainly Aβ amyloid sensors. These compounds, however, cannot be used for detecting amyloid deposition in peripheral nerves because of the inherent sensitivity of the compounds. These compounds often generate false positives especially in the basement membrane of blood vessels in histochemical studies. To overcome these problems, we must first synthesize other styryl compounds for detecting amyloid fibrils in tissues. Methods: A wide variety of symmetrical and unsymmetrical styrylbenzene derivatives were synthesized and then these compounds were used to detect amyloid fibrils in autopsy and biopsy samples from patients with various systemic and localized forms of amyloidosis such as familial amyloidotic polyneuropathy (FAP), senile systemic amyloidosis (SSA), amyloid A (AA) amyloidosis, localized AL amyloidosis, and Alzheimer's disease. Results: 1-Methoxy-2,5-bis-styrylbenzene and 2-(2-(2-fluoroethoxy)ethoxy)ethoxy)-2,5-bis-styrylbenzene (EEEFSB) detected amyloid fibrils in both in vitro and in vivo histopathological studies. 1-Methoxy-2,5-bis-styrylbenzene also showed a high strength of fluorescence with amyloid deposition in peripheral nerves in a patient with FAP. Conclusions: 1-Methoxy-2,5-bis-styrylbenzene and EEEFSB may prove a useful tool for diagnosing amyloidosis, not only in a histochemical study but also in whole body amyloid positron emission tomography (PET) imaging.

元の言語英語
ページ(範囲)27-34
ページ数8
ジャーナルClinica Chimica Acta
436
DOI
出版物ステータス出版済み - 9 25 2014

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Amyloid Plaques
Amyloid
Deposits
Derivatives
Amyloidosis
Polyneuropathies
Peripheral Nerves
Positron emission tomography
Biopsy
Blood vessels
Basement Membrane
Positron-Emission Tomography
Blood Vessels
Autopsy
Alzheimer Disease
Fluorescence
Tissue
Imaging techniques
Sensors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

これを引用

Nakazono, M., Obayashi, K., Sasamoto, K., Tomiyoshi, K., Suenaga, G., & Ando, Y. (2014). Novel styrylbenzene derivatives for detecting amyloid deposits. Clinica Chimica Acta, 436, 27-34. https://doi.org/10.1016/j.cca.2014.04.028

Novel styrylbenzene derivatives for detecting amyloid deposits. / Nakazono, Manabu; Obayashi, Konen; Sasamoto, Kazumi; Tomiyoshi, Katsumi; Suenaga, Genki; Ando, Yukio.

:: Clinica Chimica Acta, 巻 436, 25.09.2014, p. 27-34.

研究成果: ジャーナルへの寄稿記事

Nakazono, M, Obayashi, K, Sasamoto, K, Tomiyoshi, K, Suenaga, G & Ando, Y 2014, 'Novel styrylbenzene derivatives for detecting amyloid deposits', Clinica Chimica Acta, 巻. 436, pp. 27-34. https://doi.org/10.1016/j.cca.2014.04.028
Nakazono, Manabu ; Obayashi, Konen ; Sasamoto, Kazumi ; Tomiyoshi, Katsumi ; Suenaga, Genki ; Ando, Yukio. / Novel styrylbenzene derivatives for detecting amyloid deposits. :: Clinica Chimica Acta. 2014 ; 巻 436. pp. 27-34.
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