Novel translational control through an iron-responsive element by interaction of multifunctional protein YB-1 and IRP2

Megumi Ashizuka, Takao Fukuda, Takanori Nakamura, Kanemitsu Shirasuna, Kazuhiro Iwai, Hiroto Izumi, Kimitoshi Kohno, Michihiko Kuwano, Takeshi Uchiumi

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

The eukaryotic Y-box-binding protein YB-1 functions in various biological processes, including DNA repair, cell proliferation, and transcriptional and translational controls. To gain further insight into how human YB-1 plays its role in pleiotropic functions, we here used two-hybrid screenings to identify partners of this protein; the results showed that YB-1 itself, iron-regulatory protein 2 (IRP2), and five ribosomal proteins each served as partners to YB-1. We then examined the biological effect of the interaction of YB-1 and IRP2 on translational regulation. Both in vitro binding and coimmunoprecipitation assays showed the direct interaction of YB-1 and IRP2 in the presence of a high concentration of iron. RNA gel shift assays showed that YB-1 reduced the formation of the IRP2-mRNA complex when the iron-responsive element of the ferritin mRNA 5′ untranslated region (UTR) was used as a probe. By using an in vitro translation assay using luciferase mRNA ligated to the ferritin mRNA 5′UTR as a reporter construct, we showed that both YB-1 and IRP2 inhibited the translation of the mRNA. However, coadministration of YB-1 and IRP2 proteins abrogated the inhibition of protein synthesis by each protein. An In vivo coimmunoprecipitation assay showed that IRP2 bound to YB-1 in the presence of iron and a proteasome inhibitor. The direct interaction of YB-1 and IRP2 provides the first evidence of the involvement of YB-1 in the translational regulation of an iron-related protein.

本文言語英語
ページ(範囲)6375-6383
ページ数9
ジャーナルMolecular and cellular biology
22
18
DOI
出版ステータス出版済み - 9 2002

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞生物学

フィンガープリント

「Novel translational control through an iron-responsive element by interaction of multifunctional protein YB-1 and IRP2」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル