Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isoenzyme conversion in Escherichia coli, and identification of the gene product

Yoshizumi Ishino, H. Shinagawa, K. Makino, M. Amemura, A. Nakatura

研究成果: ジャーナルへの寄稿記事

583 引用 (Scopus)

抄録

The iap gene in Escherichia coli is reponsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,644-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions. The predicted iap product contained 345 amino acids with an estimated molecular weight of 37,919. The 24-amino-acid sequence at the amino terminus showed features characteristic of a signal peptide. Two proteins of different sizes were identified by the maxicell method, one corresponding to the Iap protein and the other corresponding to the processed product without the signal peptide. Neither the isozyme-converting activity nor labeled Iap proteins were detected in the osmotic-shock fluid of cells carrying a multicopy iap plasmid. The Iap protein seems to be asociated with the membrane.

元の言語英語
ページ(範囲)5429-5433
ページ数5
ジャーナルJournal of bacteriology
169
発行部数12
出版物ステータス出版済み - 12 1 1987

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Isoenzymes
Alkaline Phosphatase
Escherichia coli
Protein Sorting Signals
Genes
Proteins
Osmotic Pressure
Amino Acid Sequence
Plasmids
Molecular Weight
Amino Acids
Membranes
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

これを引用

Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isoenzyme conversion in Escherichia coli, and identification of the gene product. / Ishino, Yoshizumi; Shinagawa, H.; Makino, K.; Amemura, M.; Nakatura, A.

:: Journal of bacteriology, 巻 169, 番号 12, 01.12.1987, p. 5429-5433.

研究成果: ジャーナルへの寄稿記事

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AU - Ishino, Yoshizumi

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AU - Nakatura, A.

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