Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino, Susumu Tsunasawa, Fumio Sakiyama, Atsuo Nakata

研究成果: Contribution to journalArticle査読

40 被引用数 (Scopus)

抄録

The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

本文言語英語
ページ(範囲)1-7
ページ数7
ジャーナルMGG Molecular & General Genetics
204
1
DOI
出版ステータス出版済み - 7 1986
外部発表はい

All Science Journal Classification (ASJC) codes

  • Genetics

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