Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles

Fumio Nakamura, Masateru Ito, Ablihito Manna, Kaoru Tamada, Masahiko Hara, Wolfgang Knoll

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

To realize the sensitive detection of DNA hybridization, DNA-modified Au nanoparticles (DNA-AuNPs) containing single-and double-stranded portions were prepared. The hybridization of a target DNA using the DNA-AuNPs on a DNA self-assembled monolayer (DNA-SAM) was monitored in situ by surface plasmon resonance (SPR) imaging. A sandwich type hybridization (DNA-SAM/target DNA/DNA-AuNP) experiment was carried out in order to enhance the hybridization signal. SPR imaging results strongly indicate that the hybridization signal is enhanced several times compared to the case of target DNA hybridization.

本文言語英語
ページ(範囲)1026-1029
ページ数4
ジャーナルJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
45
2 A
DOI
出版ステータス出版済み - 2 8 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

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