We examined the incorporation of lysine into archaeal isoprenoidal lipids of halophilic archaea, Natrinema pallidum and Halobacterium halobium using two regiospecifically deuterium-labeled derivatives, [3,3-2H2] and [6,6-2H2]lysines. The two deuterated lysines were synthesized, and the incorporation of deuterium to the lipid core was defined by 2H NMR. The results revealed that lysine is degraded to crotonoyl-CoA by the decarboxylation of carboxylate in the metabolism of halophilic archaea, much like the metabolism of lysine in aerobic bacteria; the process converts lysine to isoprenoidal lipids via the mevalonate pathway through glutaryl-CoA, crotonoyl-CoA, and acetoacetyl-CoA.
!!!All Science Journal Classification (ASJC) codes
- 化学 (全般)