Occurrence of an allosteric transition in the modification of papain withl-1-acetyl-2,3-dihydropyrrolo[2,3-b]-indole-2-carboxamide

Akinori Nagatomo, Kohta Sakai, Takeru Nose, Yasuyuki Shimohigashi, Motonori Ohno

研究成果: Contribution to journalArticle査読

抄録

When papain was reacted with l-1-acetyl-2,3-dihydropyrrolo[2,3-b]indole-2-carboxamide at pH 8.0, inactivation occurred accompanied by modification of Cys-25 in the active site. Plots of pseudo first-order rate constants against the reagent concentrations yielded an anomalous sigmoidal curve, suggesting that papain responded to this reagent in an allosteric manner. This is supported by the fact that the presence of a moderate concentration (a twenty-fold molar excess) of Nα-acetyl-l-tryptophanamide over papain accelerated the inactivation.

本文言語英語
ページ(範囲)411-413
ページ数3
ジャーナルJournal of Chromatography A
597
1-2
DOI
出版ステータス出版済み - 4 24 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 臨床生化学
  • 分子医療

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