Occurrence of Keratan Sulfate in Connective Tissues of Teleost

Makoto Ito, Tetuo Morinaga, Manabu Kitamikado

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specific action of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate. Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel. From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was identified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.

本文言語英語
ページ(範囲)1445-1452
ページ数8
ジャーナルNIPPON SUISAN GAKKAISHI
48
10
DOI
出版ステータス出版済み - 1982

All Science Journal Classification (ASJC) codes

  • 水圏科学

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