Occurrence of Keratan Sulfate in Connective Tissues of Teleost

Makoto Ito, Tetuo Morinaga, Manabu Kitamikado

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specific action of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate. Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel. From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was identified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.

元の言語英語
ページ(範囲)1445-1452
ページ数8
ジャーナルNIPPON SUISAN GAKKAISHI
48
発行部数10
DOI
出版物ステータス出版済み - 1 1 1982

Fingerprint

teleost
connective tissues
sulfates
sulfate
mackerel
skin (animal)
galactosidases
skin
cornea
carbohydrate
carbohydrates
hyaluronoglucosaminidase
Flavobacterium
glycosaminoglycans
horse
polysaccharide
fins
mammal
polysaccharides
tissue

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Occurrence of Keratan Sulfate in Connective Tissues of Teleost. / Ito, Makoto; Morinaga, Tetuo; Kitamikado, Manabu.

:: NIPPON SUISAN GAKKAISHI, 巻 48, 番号 10, 01.01.1982, p. 1445-1452.

研究成果: ジャーナルへの寄稿記事

Ito, M, Morinaga, T & Kitamikado, M 1982, 'Occurrence of Keratan Sulfate in Connective Tissues of Teleost', NIPPON SUISAN GAKKAISHI, 巻. 48, 番号 10, pp. 1445-1452. https://doi.org/10.2331/suisan.48.1445
Ito, Makoto ; Morinaga, Tetuo ; Kitamikado, Manabu. / Occurrence of Keratan Sulfate in Connective Tissues of Teleost. :: NIPPON SUISAN GAKKAISHI. 1982 ; 巻 48, 番号 10. pp. 1445-1452.
@article{b6162a89a94f4816bc3c71ed8d750859,
title = "Occurrence of Keratan Sulfate in Connective Tissues of Teleost",
abstract = "A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specific action of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate. Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel. From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was identified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.",
author = "Makoto Ito and Tetuo Morinaga and Manabu Kitamikado",
year = "1982",
month = "1",
day = "1",
doi = "10.2331/suisan.48.1445",
language = "English",
volume = "48",
pages = "1445--1452",
journal = "Nippon Suisan Gakkaishi",
issn = "0021-5392",
publisher = "Nihon Suisan Gakkai",
number = "10",

}

TY - JOUR

T1 - Occurrence of Keratan Sulfate in Connective Tissues of Teleost

AU - Ito, Makoto

AU - Morinaga, Tetuo

AU - Kitamikado, Manabu

PY - 1982/1/1

Y1 - 1982/1/1

N2 - A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specific action of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate. Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel. From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was identified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.

AB - A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specific action of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate. Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel. From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was identified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.

UR - http://www.scopus.com/inward/record.url?scp=85004485930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85004485930&partnerID=8YFLogxK

U2 - 10.2331/suisan.48.1445

DO - 10.2331/suisan.48.1445

M3 - Article

VL - 48

SP - 1445

EP - 1452

JO - Nippon Suisan Gakkaishi

JF - Nippon Suisan Gakkaishi

SN - 0021-5392

IS - 10

ER -