Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells

Isao Shibuya, Kentaro Yoshimura, Yoichi Miyamoto, Atsushi Yamada, Masamichi Takami, Tetsuo Suzawa, Dai Suzuki, Noriharu Ikumi, Fumitaka Hiura, Takahisa Anada, Osamu Suzuki, Ryutaro Kamijo

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

Implantation of octacalcium phosphate (OCP), a hydroxyapatite precursor, has been reported to induce chondrogenesis in vivo. In this study, we examined the effects of OCP on the chondrogenic differentiation of mouse chondroblastic ATDC5 cells in vitro. Contrary to our expectation, chondrogenic differentiation of ATDC5 cells evaluated by the mRNA expression of Col2a1, Acan and Col10a1 was suppressed by OCP. Among Sox9, Sox5 and Sox6, essential transcription factors for chondrogenesis, the expression of Sox6 mRNA was markedly lowered by OCP. Whereas ATDC5 cells dissolved OCP to liberate calcium and inorganic phosphorus, increased calcium or phosphate in the medium had little effect on the differentiation of these cells. Direct contact of ATDC5 cells with OCP was required to suppress the expression of Col2a1 and Sox6 mRNAs, whereas the introduction of Sox6 short interfering RNA lowered the expression of Col2a1 mRNA. On the other hand, the forced expression of Sox6 protein partially but significantly, restored the expression of Col2a1 mRNA suppressed by OCP. These results indicate that OCP suppresses the chondrogenic differentiation of ATDC5 cells, at least in part, at the Sox6 transcription level.

本文言語英語
ページ(範囲)401-412
ページ数12
ジャーナルCell and tissue research
352
2
DOI
出版ステータス出版済み - 5 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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