In recent years, a way to objective evaluation of the quality and quantity of the odor is required to resolve issues such as odor nuisance, gas explosions and poisoning gas detections. It is also required to detect and track their harmful odors, and remove their sources radically. In this study, we tried to measure odor substances by fluorescent quenching. We developed imaging film to realize optical odor sensing using fluorescence dyes and the odor gas detection system using the film and a cooled CCD camera. The system could detect gaseous odor flow and visualize shape, spread and concentration distribution of odor. In addition, we developed a multi-film and FRET probes for high sensitive and selective detection of odor. FRET probe consisted of a certain combination of fluorescence dyes such as tryptophan and vanillin. FRET probe could detect odors that could not be detected by a single fluorescence probe. Multi probe film consisted of e.g. tryptophan and quinine sulfate. Multiple information of odors could be acquired by catching image of the multi probe film with interference filters.
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