Odorant response assays for a heterologously expressed olfactory receptor

Sayako Katada, Takao Nakagawa, Hiroshi Kataoka, Kazushige Touhara

研究成果: Contribution to journalArticle査読

99 被引用数 (Scopus)

抄録

Odorant responsiveness of a mouse olfactory receptor, mOR-EG, was investigated in various heterologous cells using a variety of detection methods. Odorant-induced Ca2+ response was observed in HEK293 cells that coexpressed mOR-EG and the promiscuous G protein, Gα15. Without Gα15, a robust increase in cAMP level was observed upon odorant-stimulation in various mammalian cells. A luciferase reporter gene assay using zif268 promoter was adopted to amplify the cAMP signals. In Xenopus laevis oocytes, odorant-stimulated currents were recorded when mOR-EG cRNA was co-injected with either Gα15 or cAMP-dependent channel. These results suggest that odorant responsiveness can be monitored via a signaling pathway mediated by endogenous Gαs or transfected Gα15 in heterologous cell systems. Various functional assays for a heterologously expressed olfactory receptor reported in this study, are potentially useful for high-throughput ligand screening and functional analyses of hundreds of olfactory receptors.

本文言語英語
ページ(範囲)964-969
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
305
4
DOI
出版ステータス出版済み - 6 13 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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