Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice

Katsuhiko Hayashi, Sugako Ogushi, Kazuki Kurimoto, So Shimamoto, Hiroshi Ohta, Mitinori Saitou

研究成果: ジャーナルへの寄稿記事

363 引用 (Scopus)

抄録

Reconstitution of female germ cell development in vitro is a key challenge in reproductive biology and medicine. We show here that female (XX) embryonic stem cells and induced pluripotent stem cells in mice are induced into primordial germ cell-like cells (PGCLCs), which, when aggregated with female gonadal somatic cells as reconstituted ovaries, undergo X-reactivation, imprint erasure, and cyst formation, and exhibit meiotic potential. Upon transplantation under mouse ovarian bursa, PGCLCs in the reconstituted ovaries mature into germinal vesicle-stage oocytes, which then contribute to fertile offspring after in vitro maturation and fertilization. Our culture system serves as a robust foundation for the investigation of key properties of female germ cells, including the acquisition of totipotency, and for the reconstitution of whole female germ cell development in vitro.

元の言語英語
ページ(範囲)971-975
ページ数5
ジャーナルScience
338
発行部数6109
DOI
出版物ステータス出版済み - 11 16 2012

Fingerprint

Germ Cells
Oocytes
Ovary
Reproductive Medicine
Induced Pluripotent Stem Cells
Fertilization in Vitro
Embryonic Stem Cells
Cysts
Transplantation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • General

これを引用

Hayashi, K., Ogushi, S., Kurimoto, K., Shimamoto, S., Ohta, H., & Saitou, M. (2012). Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice. Science, 338(6109), 971-975. https://doi.org/10.1126/science.1226889

Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice. / Hayashi, Katsuhiko; Ogushi, Sugako; Kurimoto, Kazuki; Shimamoto, So; Ohta, Hiroshi; Saitou, Mitinori.

:: Science, 巻 338, 番号 6109, 16.11.2012, p. 971-975.

研究成果: ジャーナルへの寄稿記事

Hayashi, K, Ogushi, S, Kurimoto, K, Shimamoto, S, Ohta, H & Saitou, M 2012, 'Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice', Science, 巻. 338, 番号 6109, pp. 971-975. https://doi.org/10.1126/science.1226889
Hayashi, Katsuhiko ; Ogushi, Sugako ; Kurimoto, Kazuki ; Shimamoto, So ; Ohta, Hiroshi ; Saitou, Mitinori. / Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice. :: Science. 2012 ; 巻 338, 番号 6109. pp. 971-975.
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