OIP5-AS1 promotes proliferation of non-small-cell lung cancer and head and neck squamous cell carcinoma cells

Yojiro Kotake, Natsumi Matsunaga, Takahiro Wakasaki, Ryotaro Okada

研究成果: Contribution to journalArticle査読

抄録

Background/Aim: The long noncoding RNA OIP5 antisense RNA 1 (OIP5-AS1) is overexpressed in various cancer types, such as lung cancer, hepatoblastoma and cervical cancer, and functions to accelerate cell proliferation, invasion and migration. Here, we investigated the roIe of OIP5-AS1 in cell-cycle progression of H1299 and A549 non-small cell lung cancer cells, and FaDu and CAL27 head and neck squamous cell carcinoma cells. Materials and Methods: The cells were transfected with small interfering RNA and subjected to cell-cycle analysis and reverse-transcription quantitative polymerase chain reaction (RT-qPCR). Results: Silencing of OIP5-AS1 suppressed the proliferation of H1299, A549, FaDu and CAL27 cells. RT-qPCR and cell-cycle analysis revealed that silencing OIP5-AS1 increased the expression of CDK inhibitors, such as p15, p16, p18 and p19, resulting in G1-phase arrest. Conclusion: OIP5-AS1 regulates G1-phase progression by repressing CDK inhibitors and, thus, promotes the proliferation of H1299, A549, FaDu and CAL27 cells.

本文言語英語
ページ(範囲)543-548
ページ数6
ジャーナルCancer Genomics and Proteomics
18
4
DOI
出版ステータス出版済み - 7 2021

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 遺伝学
  • 癌研究

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