Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference

Takahisa Anada, Masako Ogawa, Hisashi Yokomizo, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant was detected as usual. In contrast, when the 6-mer ODN was used as the affinity ligand with a mixture of the complementary ODN and its single-base mutant, it was possible to detect both as completely separate peaks. That is, the separation mode was dependent on the base number of the immobilized ODN used as an affinity ligand.

元の言語英語
ページ(範囲)73-77
ページ数5
ジャーナルAnalytical Sciences
19
発行部数1
DOI
出版物ステータス出版済み - 1 1 2003

Fingerprint

Oligodeoxyribonucleotides
Single-Stranded DNA
Ligands
Capillary electrophoresis
Genes
DNA

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference. / Anada, Takahisa; Ogawa, Masako; Yokomizo, Hisashi; Ozaki, Yoshihisa; Takarada, Tohru; Katayama, Yoshiki; Maeda, Mizuo.

:: Analytical Sciences, 巻 19, 番号 1, 01.01.2003, p. 73-77.

研究成果: ジャーナルへの寄稿記事

@article{5991ea52457f4b65b72495e561639108,
title = "Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference",
abstract = "We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant was detected as usual. In contrast, when the 6-mer ODN was used as the affinity ligand with a mixture of the complementary ODN and its single-base mutant, it was possible to detect both as completely separate peaks. That is, the separation mode was dependent on the base number of the immobilized ODN used as an affinity ligand.",
author = "Takahisa Anada and Masako Ogawa and Hisashi Yokomizo and Yoshihisa Ozaki and Tohru Takarada and Yoshiki Katayama and Mizuo Maeda",
year = "2003",
month = "1",
day = "1",
doi = "10.2116/analsci.19.73",
language = "English",
volume = "19",
pages = "73--77",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "The Japan Society for Analytical Chemistry",
number = "1",

}

TY - JOUR

T1 - Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference

AU - Anada, Takahisa

AU - Ogawa, Masako

AU - Yokomizo, Hisashi

AU - Ozaki, Yoshihisa

AU - Takarada, Tohru

AU - Katayama, Yoshiki

AU - Maeda, Mizuo

PY - 2003/1/1

Y1 - 2003/1/1

N2 - We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant was detected as usual. In contrast, when the 6-mer ODN was used as the affinity ligand with a mixture of the complementary ODN and its single-base mutant, it was possible to detect both as completely separate peaks. That is, the separation mode was dependent on the base number of the immobilized ODN used as an affinity ligand.

AB - We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant was detected as usual. In contrast, when the 6-mer ODN was used as the affinity ligand with a mixture of the complementary ODN and its single-base mutant, it was possible to detect both as completely separate peaks. That is, the separation mode was dependent on the base number of the immobilized ODN used as an affinity ligand.

UR - http://www.scopus.com/inward/record.url?scp=0037269520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037269520&partnerID=8YFLogxK

U2 - 10.2116/analsci.19.73

DO - 10.2116/analsci.19.73

M3 - Article

C2 - 12558027

AN - SCOPUS:0037269520

VL - 19

SP - 73

EP - 77

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 1

ER -