On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

元の言語英語
記事番号5299057
ページ(範囲)312-317
ページ数6
ジャーナルIEEE Transactions on Nanobioscience
8
発行部数4
DOI
出版物ステータス出版済み - 12 1 2009

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Cell immobilization
Immobilization
Gels
Yeast
Monitoring
Yeasts
Micromachining
Gelation
Microtechnology
Microchannels
Tin oxides
Microfluidics
Hydrogels
Indium
Hydrogel
Dyes
Fluorescent Dyes
Cells
Fabrication
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

これを引用

On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge. / Yamanishi, Yoko; Teramoto, Jun; Magariyama, Yukio; Ishihama, Akira; Fukuda, Toshio; Arai, Fumihito.

:: IEEE Transactions on Nanobioscience, 巻 8, 番号 4, 5299057, 01.12.2009, p. 312-317.

研究成果: ジャーナルへの寄稿記事

Yamanishi, Yoko ; Teramoto, Jun ; Magariyama, Yukio ; Ishihama, Akira ; Fukuda, Toshio ; Arai, Fumihito. / On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge. :: IEEE Transactions on Nanobioscience. 2009 ; 巻 8, 番号 4. pp. 312-317.
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AU - Arai, Fumihito

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